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Flow-through fluorocytophotometry of different brain tumours

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Summary

The flow-through cytophotometric method was used to investigate the single-cell DNA content in 60 tumours of the CNS and allied structures (9 meningiomas, 5 ependymomas, 15 astrocytomas, 11 anaplastic astrocytomas, 8 glioblastomas, 7 medulloblastomas, 2 oligodendrogliomas, 1 anaplastic oligodendroglioma, and 2 neurinomas). The cytophotometric parameters were correlated with morphological and clinical data of the tumours. The results are summarised in Tables 1–7. With regard to the DNA content of their cell nuclei, the gliomas present a behaviour similar to tumours in other organs, but in the present study the cytophotometric signs of malignancy in gliomas are not so evident as in carcinomas or sarcomas. The information obtained by flow cytophotometric methods may be helpful in diagnosing the degree of malignancy in brain tumours.

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References

  • Andreeff, M.: Impulscytophotometrie. Berlin, Heidelberg, New York: Springer 1975

    Google Scholar 

  • Arendt, A.: Die gewebliche Entdifferenzierung der Gliome. Oncologia17, 16–30 (1964)

    Google Scholar 

  • David, E., Unger, R. R.: Zur Problematik maligne entarteter Gliome des Großhirns (Teil II). Arch. Geschwulstforsch.30, 135–141 (1967)

    Google Scholar 

  • Dilla, M. A. van, Trujillo, T. T., Mullancy, P. F., Coulter, J. R.: Cell microfluorometry: A method for rapid fluorescence measurements. Science163, 1213–1214 (1969)

    Google Scholar 

  • Dittrich, W., Göhde, W.: Impulscytophotometrie bei Einzelzellen in Suspensionen. Z. Naturforsch. [C]24b, 360–361 (1969)

    Google Scholar 

  • Frederiksen, P., Reske-Nielsen, E., Bichel, P.: Flow cytometry in tumours of the brain. Acta Neuropathol. (Berl.)41, 179–183 (1978)

    Google Scholar 

  • Frederiksen, P., Reske-Nielsen, E., Bichel, P.: DNA content of meningiomas. Acta Neuropathol. (Berl.)46, 65–68 (1979)

    Google Scholar 

  • Gerstner, L., Jellinger, K., Heiss, W.-D., Wöber, G.: Morphological changes in anaplastic gliomas treated with radiation and chemotherapy. Acta Neurochir. (Wien)36, 117–138 (1977)

    Google Scholar 

  • Horan, P. K., Wheeless, L. L., Jr.: Quantitative single cell analysis and sorting. Science198, 149–157 (1977)

    Google Scholar 

  • Hoshino, T., Barker, M., Wilson, C. B., Boldrey, E., Fewer, D.: Cell kinetics in human gliomas. J. Neurosurg.37, 15–26 (1972)

    Google Scholar 

  • Hoshino, T., Wilson, C. B., Rosenblum, M. L., Barker, M.: Chemotherapeutic implications of growth fraction and cell cycle time in glioblastomas. J. Neurosurg.43, 127–135 (1975)

    Google Scholar 

  • Hoshino, T., Nomura, K., Gray, J. W., Wilson, L. B.: Flow microfluorometric studies of brain and brain tumors. J. Neuropathol. Exp. Neurol.35, 363 (1976)

    Google Scholar 

  • Hoshino, T., Nomura, K., Wilson, C. B., Knebel, K. D., Gray, J. W.: The distribution of nuclear DNA from human brain tumor cells. J. Neurosurg.49, 13–21 (1978)

    Google Scholar 

  • Kajikawa, H., Kawamoto, K., Herz, F., Wolley, R. C., Hirano, A., Koss, L. G.: Flow-through cytometry of meningiomas and cultured meningioma cells. Acta Neuropathol. (Berl.)44, 183–187 (1978)

    Google Scholar 

  • Kamentsky, L. A., Melamed, M. R., Derman, H.: Spectrophotometer: New instrument for ultrarapid cell analysis. Science150, 630–631 (1965)

    Google Scholar 

  • Kawamoto, K., Herz, F., Wolley, R. C., Hirano, A., Kajikawa, H., Koss, L. G.: Flow cytometric analysis of the DNA distribution in human brain tumors. Acta Neuropathol. (Berl.)46, 39–44 (1979)

    Google Scholar 

  • Khominsky, B. S., Brodskaya, I. A.: Histochemistry of nucleic acids and proteins in cells of neuroectodermal tumours of different grades of malignancy. Neoplasma20, 281–296 (1973)

    Google Scholar 

  • Kortmann, G.: Cytophotometrische Untersuchungen über den DNS-Gehalt in den Zellkernen des Glioblastoms. Inaug.-Diss., Univ. Köln 1969

  • Krug, H.: Die Impulszytophotometrie als quantitative Methode der Zytochemie. Acta Histochem. (Jena) [Suppl.]15, 377–395 (1975)

    Google Scholar 

  • Krug, H.: Impulszytophotometrische Untersuchungen an Tumormaterial. Arch. Geschwulstforsch.46, 110–115 (1976a)

    Google Scholar 

  • Krug, H.: Ein mathematisches Modell des Zusammenhangs von DNS-Karyogramm und Zellzyklus. 1. Mitteilung: Die Ableitung der DNS-Häufigkeitsverteilung aus der Dauer der Einzelphasen des Zellzyklus. Acta Histochem. (Jena)56, 140–155 (1976b)

    Google Scholar 

  • Krug, H., Ebeling, K.: Pulse cytophotometric investigations concerning ploidy and proliferation pattern of invasive squamous cell carcinomas of the cervix uteri. Exp. Pathol. (Jena)13, 237–246 (1977)

    Google Scholar 

  • Krug, H., Schönfelder, M.: Impulszytophotometrische Untersuchungen an Melanoblastomen. Arch. Geschwulstforsch.45, 347–367 (1975)

    Google Scholar 

  • Lapham, L. W.: Subdivision of glioblastoma multiforme on a cytologic and cytochemical basis. J. Neuropathol. Exp. Neurol.18, 244–262 (1959)

    Google Scholar 

  • Melamed, M. R., Kamentsky, L. A.: Automated cytology. Int. Rev. Exp. Pathol.14, 205–295 (1975)

    Google Scholar 

  • Müller, W.: DNA estimations in cerebral tumours of man. Neuropatol. Pol.10, 121–128 (1972)

    Google Scholar 

  • Müller, W.: DNA-pattern in gliomas of grades I–III. In: Modern aspects of neurosurgery. Proceedings of the German Society for Neurosurgery. Vol. 3, pp. 125–126. Amsterdam: Excerpta Medica 1973

    Google Scholar 

  • Müller, W.: The glioblastoma in respect to the DNA distribution pattern. In: VIIth International Congress of Neuropathology, Budapest 1974, Proceedings, pp. 545–547, Környey, S., Tariska, S., Gosztonyi, G. (eds.). Budapest: Akadémiai Kiadó 1975

    Google Scholar 

  • Müller, W., Brämisch, R., Afra, D., Schwenzfeger, A.: Cytophotometrische Messungen des DNS-Gehaltes in Ependymomen und Plexuspapillomen. Acta Neuropathol. (Berl.)39, 255–259 (1977)

    Google Scholar 

  • Patzer, P.: Über den DNS-Gehalt in den Zellkernen des menschlichen Medulloblastoms. Inaug.-Diss., Univ. Köln 1969

  • Pera, F.: Mechanismen der Polyploidisierung und der somatischen Reduktion. Ergeb. Anat. Entwicklungsgesch.43, 1–112 (1970)

    Google Scholar 

  • Pevzner, L. Z., Tomina, E. D., Chaika, T. V.: Cytospectrophotometric study of DNA content in the cells of human brain tumours. Vopr. Med. Khim.10, 379–386 (1964)

    Google Scholar 

  • Scarlato, G., Müller, W.: Quantitative histochemische Untersuchungen des Desoxyribonukleinsäure-Gehaltes in Hirntumoren. Zentralbl. Neurochir.19, 179–185 (1959)

    Google Scholar 

  • Stolpmann, R.: Zytophotometrische DNS-Messungen an isolierten Zellkernen des Medulloblastoms. Inaug.-Diss., Univ. Köln 1968

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Authors and Affiliations

  1. Department of Neuropathology, Institute of Pathology, Karl-Marx-University of Leipzig, Liebigstr. 26, DDR-701, Leipzig, German Democratic Republic

    J. Lehmann

  2. Department of Histophysics and Cytophotometry, Institute of Pathology, Karl-Marx-University of Leipzig, Liebigstr. 26, DDR-701, Leipzig, German Democratic Republic

    H. Krug

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  1. J. Lehmann
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  2. H. Krug
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Lehmann, J., Krug, H. Flow-through fluorocytophotometry of different brain tumours. Acta Neuropathol 49, 123–132 (1980). https://doi.org/10.1007/BF00690752

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  • DOI: https://doi.org/10.1007/BF00690752

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