Summary
Observations were made between the ages of 2 and 12 months on rats made diabetic with streptozotocin at the age of 1 month, and compared with the findings in age-matched controls. Tibial length and body weight in the control animals increased progressively over the period examined, the growth rate being more rapid in the initial stages. Both of these parameters were consistently less in the diabetic animals over the whole of the observation period. Myelinated fibre numbers and diameters were measured in the tibial and plantar nerves. In the tibial nerve, fibre diameter did not differ between the diabetic and control animals up until 4 months of age; thereafter it changed little in the diabetic animals, but continued to increase in the controls. The findings in the medial plantar nerve were more difficult to analyse but showed comparable although less pronounced changes; fibre diameter may be have diminished in the diabetic nerves after 6 months. Teased fibre studies demonstrated few abnormalities in the tibial nerve, either in the control or the diabetic rats. In the lateral plantar nerves, there was a significant excess of axonal degeneration and regeneration in the diabetic nerves.
It was concluded that diabetes impairs growth in nerve fibre diameter, but only after 4 months of age. Before then, no growth retardation is obvious, despite the fact that tibial length and body weight are less. This suggests that the peripheral nervous system may be protected against growth retardation during the early part of the postnatal growth period. The significance of the axonal degeneration in the plantar nerves is uncertain, but it may represent either an increased vulnerability of diabetic nerve to compression injury or, less probably, a distal axonopathy related to the diabetic state.
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References
Bajada S, Sharma AK, Thomas PK (1980) Axoplasmic transport in vagal afferent fibres in normal and alloxan diabetic rabbits. J Neurol Sci 47:365–378
Bisby MA, Bulger VT (1977) Reversal of axonal transport at a nerve crush. J Neurochem 29:313–320
Brown MJ, Sumner AJ, Greene DA, Diamond SM, Asbury AK (1980) Distal neuropathy in experimental diabetes mellitus. Ann Neurol 8:168–178
Chopra JS, Sawhney BB, Chakravorty RN (1977) Pathology and time relationship of peripheral nerve changes in experimental diabetes. J Neurol Sci 32:53–67
Eliasson SG (1964) Nerve conduction changes in experimental diabetes. J Clin Invest 43:2353–2358
Grover-Johnson N, Spencer PS (1979) Peripheral nerve abnormalities in aging rats (abstract). J Neuropath Exp Neurol 38:316
Hildebrand J, Joffroy J, Graff G, Coërs C (1968) Neuromuscular changes with alloxan hyperglycemia. Electrophysiological, biochemical and histological study in rats. Arch Neurol (Chic) 18:633–641
Jakobsen J (1976a) Axonal dwindling in early experimental diabetes. I. A study of cross-sectioned nerves. Diabetologia 12:539–546
Jakobsen J (1976b) Axonal dwindling in early experimental diabetes. II. A study of isolated nerve fibres. Diabetologia 12:547–553
Jakobsen J, Lundback K (1976) Neuropathy in experimental diabetes. An animal model. Br Med J 2:278–279
Jakobsen J, Sidenius P (1979) Decreased axonal flux of retrogradely transported glycoproteins in early experimental diabetes. J Neurochem. 33:1055–1060
Jakobsen J, Sidenius P (1980) Decreased axonal transport of structural proteins may explain the reduction in axon caliber and conduction velocity in early experimental diabetes. Neurology (Minneap) 30:434
Jefferys JGR, Brismar T (1980) Analysis of peripheral nerve function in streptozotocin diabetic rats. J Neurol Sci 48:435–444
Morgane FJ, Miller M, Kempner T, Stern W, Forbes W, Hall R, Bronzino J, Kissane J, Hawrylewicz E, Resnick O (1978) The effect of protein-caloric malnutrition on the developing nervous system in the rat. Neurosci Biobehav Rev 2:137–230
Mulder DW, Lambert EH, Bastron JA, Sprague RG (1961) The neuropathies associated with diabetes mellitus: a clinical and electromyographic study of 103 unselected diabetic patients. Neurology (Minneap) 11:275
Nordborg C (1975) The influence of protein-caloric malnutrition on the development of internodal segments in peripheral nerve and spinal roots. An experimental morphometric and lipid histochemical study on rats. Thesis, University of Göteborg
Oldfors A, Ullman M (1980) Motor nerve conduction velocity and nerve fibre diameter in experimental protein deprivation. Studies on rat peripheral nerve during development. Acta Neuropathol (Berl) 51:215–221
Powell H, Knox D, Lee S, Charters AC, Orloff M, Garrett R, Lampert P (1977) Alloxan diabetic neuropathy: electron microscopic studies. Neurology (Minneap) 27:60–66
Powell HC, Ward HE, Garrett RS, Orloff MJ, Lampert PW (1979) Glycogen accumulation in the nerves and kidney of chronically diabetic rats. A quantitative electron microscope study. J Neuropath Exp Neurol 38:114–127
Preston GM (1967) Peripheral neuropathy in the alloxan-diabetic rat. J Physiol (Lond) 189:49P
Schmidt RE, Matschinsky FM, Godfrey DA, Williams AD, McDougal DB Jr (1975) Fast and slow axoplasmic flow in sciatic nerve of diabetic rats. Diabetes 24:1081–1085
Seneviratne KN, Peiris OA (1969) The effects of hypoxia on the excitability of the isolated peripheral nerves of the alloxandiabetic rats. J Neurol Neurosurg Psychiat 32:462–469
Sharma AK, Thomas PK (1974) Peripheral nerve structure and function in experimental diabetes. J Neurol Sci 23:1–15
Sharma AK, Thomas PK, Baker RWR (1976) Peripheral nerve abnormalities related to galactose administration in rats. J Neurol Neurosurg Psychiat 39:794–802
Sharma AK, Thomas PK, De Molina AF (1977) Peripheral nerve fiber size in experimental diabetes. Diabetes 26:689–692
Sharma AK, Bajada S, Thomas PK (1980) Age changes in the tibial and plantar nerves of the rat. J Anat 130:417–428
Sigimura K, Windebank A, Natarajan V, Lambert E, Schmid H, Dyck PJ (1980) Interstitial hyperosomolarity may cause axis cylinder shinkage in streptozotocin-diabetic nerves. Neurology (Minneap) 30:434
Thomas PK (1955) Growth changes in the myelin sheaths of peripheral nerve fibres in fishes. Proc Roy Soc B 143:380–391
Thomas PK, Jefferys JGR, Sharma AK, Bajada S (1981) Nerve conduction velocity in experimental diabetes in the rat and rabbit. J Neurol Neurosurg Psychiat (in press)
Thomas PK, King RHM, Sharma AK (1980) Changes with age in the peripheral nerves of the rat: an ultrastructural study. Acta Neuropathol (Berl) 52:1–6
Vizoso AD, Young JZ (1948) Internode length and fibre diameter in developing and regnerating nerves. J Anat 82:110–134
Waxman SG (1980) Determinants of conduction velocity in myelinated nerve fibers. Muscle Nerve 3:141–150
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Sharma, A.K., Bajada, S. & Thomas, P.K. Influence of streptozotocin-induced diabetes on myelinated nerve fibre maturation and on body growth in the rat. Acta Neuropathol 53, 257–265 (1981). https://doi.org/10.1007/BF00690367
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DOI: https://doi.org/10.1007/BF00690367