Summary
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1.
The activities of octopine dehydrogenase (ODH) and α-glycerophosphate dehydrogenase (GDH) were determined in several tissues ofPecten maximus. The ratios of ODH/GDH were 13 for the adductor muscle, 9 for the mantle and 3 for the digestive gland and the gill. Therefore, the main pathway for the glycolytic re-oxidation of NADH seemed to be via the formation of octopine.
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2.
During burst activity the level of arginine phosphate in the adductor muscle declined concurently with an increase in arginine. Little octopine, however, accumulated until the animals were exhausted; the main increase in octopine concentration took place during the beginning of the subsequent rest period.
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3.
It is concluded that inPecten maximus there is no “flare-up” of glycolysis during burst activity. Instead the increased demand for ATP is met by the breakdown of arginine phosphate. Octopine accumulates later, possibly due to a stimulation of glycolysis brought about by the activation of phosphofructokinase by AMP at low levels of arginine phosphate.
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Abbreviations
- GDH:
-
α-glycerophosphate dehydrogenase (E.C.1.1.1.8)
- ODH:
-
octopine dehydrogenase (E.C.1.5.1.11)
- LDH:
-
lactate dehydrogenase (E.C.1.1.1.27)
- DTT:
-
dithiothreitol
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Gäde, G., Weeda, E. & Gabbott, P.A. Changes in the level of octopine during the escape responses of the scallop,Pecten maximus (L.). J Comp Physiol B 124, 121–127 (1978). https://doi.org/10.1007/BF00689172
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DOI: https://doi.org/10.1007/BF00689172