Summary
Based on enzyme histochemical findings in intracranial and intraspinal meningiomas, the topic pattern of the following enzymes in these tumours is reported: acid and alkaline phosphatase, arylesterase, leucine aminopeptidase, tetrazolium salt-reductase (NAD), succinic dehydrogenase and cytochrome oxydase. As to the localization of alkaline phosphatase, the examined tumours can be divided into three groups: 1. enzyme activity in the vessel walls exclusively, 2. positive reaction only in the cytoplasm of the tumour cells, 3. enzyme activity both in vessel walls and cytoplasm. Alkaline phosphatase localized in the vessel walls is considered to be of another substrate specifity than that in cytoplasm. Tetrazolium salt-reductase (NAD) is not only to be found in cytoplasm but often also in nuclear inclusions which are probably identical with nuclear membran invaginations as seen with the electron microscope. Lactate dehydrogenase in meningiomas is also present in soluble form; therefore the topochemical identification of this enzyme is not possible by means of the usual methods.
Zusammenfassung
Auf Grund fermenthistochemischer Untersuchungen an intrakraniellen und intraspinalen Meningiomen wird über die Verteilung folgender Enzyme in diesen Geschwülsten berichtet: saure und alkalische Phosphatase, Arylesterase, Leucinaminopeptidase, Tetrazoliumsalzreduktase (NAD), Succinatdehydrogenase und Cytochromoxydase. Hinsichtlich der Lokalisation der alkalischen Phosphatase lassen sich die untersuchten Tumoren in folgende 3 Gruppen einteilen: 1. Enzymaktivität ausschließlich in den Gefäßwänden, 2. nur das Cytoplasma der Tumorzellen positiv, 3. sowohl Cytoplasma als auch Gefäßwände zeigen Fermentreaktion. Die alkalische Phosphatase, die in den Gefäßwänden lokalisiert ist, besitzt vermutlich eine andere Substratspezifität als die im Cytoplasma. Tetrazoliumsalzreduktase (NAD) ist außer im Cytoplasma auch in den meisten Fällen in Kerneinschlüssen zu erkennen, die vermutlich mit den elektronenmikroskopisch beobachteten Kernwandinvaginationen identisch sind. Abschließend wird gezeigt, daß die Lactatdehydrogenase auch in den Meningiomen in löslicher Form vorliegt und somit ein topochemischer Nachweis dieses Enzyms mit den bisher üblichen Methoden nicht möglich ist.
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Osske, G., Jänisch, W. Zur Enzymhistochemie der Meningiome. Acta Neuropathol 9, 290–297 (1967). https://doi.org/10.1007/BF00687938
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DOI: https://doi.org/10.1007/BF00687938