Summary
The selective toxicity of silica dust for macrophages has been used to assess the role of these cells in experimental allergic neuritis (EAN). Inbred Lewis rats were inoculated with bovine dorsal roots in Freund's complete adjuvant (day 0). In two experiments, animals received 200 mg of silica dust in 1 cm3 of saline intraperitoneally (IP) at days 8 and 16. In another two experiments, animals received IP silica at days 3, 7, and 11. Control animals received 1 cm3 saline IP at corresponding times. Regular clinical assessment showed that in animals treated on days 8 and 16 there was a significant delay in the time taken to reach their maximum degree of illness. This delay was not seen in the animals treated on days 3, 7, and 11. Neither of the injection regimes reduced the final maximum severity of the disease. In three experiments recovery of the treated and control animals occurred concurrently, hence the duration of the disease was reduced in the animals treated at days 8 and 16. However, in one group of animals given silica at days 3, 7 and 11, there was a delay in the time taken to recover from the most severe phase of the disease but thereafter the treated animals improved more quickly to reach their best grade at the same time as the controls. If the silica blockade of macrophages is to be effective in delaying the onset of EAN, the timing of injections is critical.
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References
Baur PS, Stacey TR (1977) The use of PIPES buffer in the fixation of mammalian and marine tissues for electron microscopy. J Microsc 109:315–327
Ballin RHM, Thomas PK (1969) Electron microscope observations on demyelination and remyelination in experimental allergic neuritis. Part 1. Demyelination. J Neurol Sci 8:1–18
Brosnan CF, Bornstein MB, Bloom BR (1981) The effects of macrophage depletion on the clinical and pathologic expression of experimental allergic encephalomyelitis. J Immunol 126:614–620
Carlo DJ, Karkhanis YD, Bailey PJ, Wiśniewski HM, Brostoff SW (1975) Experimental allergic neuritis: evidence for the involvement of the P0 and P2 proteins. Brain Res 88:580–584
Caspary EA, Field EJ (1965) Antibody response to central and peripheral nerve antigens in rat and guinea pig. J Neurol Neurosurg Psychiatry 28:179–182
Craggs R, King RHM (1983) Alteration of experimental allergic neuritis (EAN) by silica blockade of macrophage function. Neuropathol Appl Neurobiol 9:73
Dal Canto MC, Johnson AB, Raine CS, Wiśniewski HM, Brostoff SW (1974) Experimental allergic neuritis: cells binding horseradish conjugates of myelin basic proteins. J Immunol 113:387–394
Dalley BK, Selinger WG (1980) A new technique for rapid screening and selection of large pieces of tissue for ultrastructural evaluation. Stain Technol 55:129–136
Gibson JD (1979) The origin of the neural macrophage: a quantitative ultrastructural study of cell population changes during Wallerian degeneration. J Anat 129:1–19
Gross MLP, Craggs RI, King RHM, Thomas PK (1983) The treatment of experimental allergic neuritis by plasma exchange. J Neurol Sci 61:149–160
Karnovsky MJ (1965) A formaldehyde — glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A
King RHM, Craggs RI, Gross MLP, Tompkins C, Thomas PK (1983) Suppression of experimental allergic neuritis by Cyclosporin A. Acta Neuropathol (Berl) 59:262–268
Lampert PW (1969) Mechanism of demyelination in experimental allergic neuritis. Lab Invest 20:127–138
Langford LA, Coggeshall RE (1980) The use of potassium ferricyanide in neural fixation. Anat Rec 197:297–303
Morris JH, Hudson AR, Weddell G (1972) A study of degeneration and regeneration in the divided rat sciatic nerve based on electron microscopy. Z Zellforsch Mikrosk Anat 124:76–102
Pepys MD (1979) Experimental allergic neuritis In: Rose FC (ed) Clinical neuroimmunology. Blackwell. Oxford, pp 155–164
Rosenberg M, Bartl P, Leško J (1960) Water-soluble methacrylate as an embedding medium for the preparation of ultrathin sections. J Ultrastruct Res 4:298–303
Saida T, Saida K, Silberberg DH, Brown MJ (1978) Transfer of demyelination by intraneural injection of experimental allergic neuritis serum. Nature 272:639–641
Schröder JM, Krücke W (1970) Zur Feinstruktur der experimentell allergischen Neuritis beim Kaninchen. Acta Neuropathol (Berl) 14:261–283
Sievers J (1971) Basic two-dye stains for epoxy-embedded 0.3–1 μm sections. Stain Technol 46:195–199
Sobue G, Yamato S, Hirayama M, Matsuoka Y, Uematsu H, Sobue I (1982) The role of macrophages in demyelination in experimental allergic neuritis. J Neurol Sci 56:75–87
Steinman L, Rosenbaum JT, Sriram S, McDevitt HO (1981) In vivo effects of antibodies to immune response gene products —prevention of experimental allergic encephalitis. Proc Natl Acad Sci (USA) 78:7111–7114
Tansey FA, Brosnan CF (1982) Protection against experimental allergic neuritis with silica quartz dust. J Neuroimmunol 3:169–179
Waksman BH, Adams RD (1955) Allergic neuritis: an experimental disease of rabbits induced by injection of peripheral nervous tissue and adjuvants. J Exp Med 102:213–234
Waksman BH, Adams RD (1956) A comparative study of experimental allergic neuritis in rabbit, guinea pig and mouse. J Neuropathol Exp Neurol 15:293–314
Wiśniewski HM, Prineas J, Raine CS (1969) An ultrastructural study of experimental demyelination and remyelination. Part 1: Acute experimental allergic encephalomyelitis in the peripheral nervous system. Lab Invest 21:105–118
Yonezawa T, Ishihara T, Matsuyama H (1968) Studies on experimental allergic peripheral neuritis. Part 1: Demyelinating patterns studied in vitro. J Neuropathol Exp Neurol 27:453–463
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Craggs, R.I., King, R.H.M. & Thomas, P.K. The effect of suppression of macrophage activity on the development of experimental allergic neuritis. Acta Neuropathol 62, 316–323 (1984). https://doi.org/10.1007/BF00687614
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DOI: https://doi.org/10.1007/BF00687614