Summary
Herpes simplex virus was inoculated intradermally into the pad of the hind leg and intramuscularly into the gastrocnemius muscle of twelve-day-old suckling mice. The development of the resulting lesions in the sciatic nerve, spinal roots, dorsal ganglia and spinal cord was studied. After intradermal inoculations the lesions followed the sensory fibres, while after intramuscular inoculation lesions corresponding to the motor fibres were seen as a prominent feature. Ultrastructurally herpes simplex virus-like particles were seen in Schwann cells and neurones in these fibre tracts. It is reasonable to suppose that a main mechanism for the neural spread of herpes simplex virus in mice is a direct transport of the infectious agent within axons.
Zusammenfassung
Herpes simplex-Virus wurde intradermal in die Pfote des Hinterbeines sowie i.m. in den M. gastrocnemius von 12 Tage alten Saugmäusen inoculiert. Die Entwicklung der resultierenden Läsionen im Nervus ischiadicus, in den Spinalwurzeln, Spinalganglien und im Rückenmark wurde untersucht. Nach intradermaler Inoculation folgten die Läsionen den sensorischen Fasern, während nach intramuskulärer Inoculation sich hauptsächlich Läsionen in motorischen Faserm fanden. Ultramikroskopisch waren Herpes simplex-Virus-ähnliche Partikel in Schwann-Zellen und Nervenzellen dieser Faserbündel zu sehen. Die Annahme ist begründet, daß ein Hauptmechanismus für die neurale Ausbreitung des Herpes simplex-Virus ein direkter Transport des Infektionserregers in den Axonen ist.
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This study was supported by a grant, B69-12X-82-05, from the Swedish Medical Research Council. The author wishes to express his gratitude to Assoc. Prof. P. Sourander and Ass. Prof. E. Lycke for valuable criticism and advice and to Miss Margareta Persson for skilful technical assistance.
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Kristensson, K. Morphological studies of the neural spread of herpes simplex virus to the central nervous system. Acta Neuropathol 16, 54–63 (1970). https://doi.org/10.1007/BF00686964
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DOI: https://doi.org/10.1007/BF00686964