Summary
Immunoperoxidase techniques were used to obtain information about the possible presence of serum factors in senile plaques. We found only in plaques consisting of an amyloid core surrounded by a corona of degenerating neurites small amounts of IgG and light chains (kappa and labda). These immunoglobulins were principally localized in the corona and not in the central amyloid core. further it was found that all plaques contain the complement factors C1q, C3b, C3c, C3d and C4. Senile plaques lacked C5, C3 pro-activator and properdin. The possible significance of these findings in the gensis of the senile plaques and amyloid formation is discussed.
This is a preview of subscription content, log in via an institution to check access.
References
Avrameas S, Ternynck T (1971) Peroxidase labeled antibody and Fab conjugate with enhanced intracellular penetration. Immunochemistry 8:1175–1179
Boorsma DM, Streefkerk JG (1978) Improved method for separation of peroxidase conjugates. In: Knapp W, Holubar, K, Wick G (eds) Immunofluorescence and related staining techniques. Elsevier-North Holland Biochemical Press Amsterdam, pp 225–235
Boorsma DM, Streefkerk JG (1976a) Some aspects of the preparation, analysis and use of peroxidase-antibody conjugates in immunohistochemistry. In: Pecters H (ed) Protides of the biological fluids, vol 24. Pergamon Press, Oxford, pp 795–802
Boorsma DM, Streefkerk JG (1976b) Peroxidase conjugate chromotography. Isolation of conjugates prepared with glutaraldehyde of periodate using polyacrylamidelagarose gel. J Histochem Cytochem 24:481–486
Davis BJ, Ornstein L (1959) High resolution enzyme localization with a new diazo reagent, “hexazonium Pararosaniline”. J Histochem Cytochem 7:297–298
Divry P (1927) Etude histochimique des plaques seniles. J Belge Nerol 27:643–657
Du Clos TW, Mold C, Paterson PY, Alroy J, Gewurz H (1981) Localization of C-reactive protein in inflammatory lesions of experimental allergic encephalomyelitis. Clin Exp Immunol 43:565–573
Gewurz H, Lint TF (1977) Alternative modes and pathways of complement activation. In: Day NK, Good RA (eds) Biological amplification systems in immunology. Plenum Med Book, New York, pp 17–45
Glenner GG (1978) Current knowledge of amyloid deposits as applied to senile plaques and congophilic angiopathy. In: Katzman R, Terry RD, Bick KL (eds) Alzheimer's disease and related disorders. Raven Press, New York, pp 493–501
Ishii T, Haga S (1976) Immuno-electron microscopic localization of immunoglobulins in amyloid fibrils of senile plaques. Acta Neuropathol (Berl) 36:243–249
Ishii T, Haga S, Shimuzu F (1975) Identification of components of immunoglobulins in senile plaques by means of fluorescent antibody technique. Acta Neuropathol (Berl) 32:157–162
Kaplan MH, Volanakis JE (1974) Intraction of C-ractive protein complexes with the complement system. I. Consumption of human complement associated with the reaction of C-reactive protein with pneumococcal C-polysaccharide and with choline phosphatides, leithin and sphingomyelin. J Immunol 112:2135–2147
Katenkamp D, Stiller D, Thoss K (1970) Untersuchungen zum immunohistochemischen Verhalten der senilen Plaques des menschlichen Gehirnes. Virchows Arch A [Pathol Anat] 351:333–339
Linder E (1980) Similarities between cytoskeletal “intermediate” 10 nm filaments and amyloid. In: Glenner GG, Pinho E, Costa P, Falcao de Freitas A (eds) Amyloid and amyloidosis. Excerpta Medica, Amsterdam, pp 283–287
Linder E, Lento VP, Stenman S (1979) Activation of complement by cytoskeletal intermediate filaments. Nature 278:176–178
Pearse AGE (1968) Histochemistry. Theoretical and applied, vol II, 3rd edn. Churchill Livingstone, Edinburgh
Powers JM, Skeen JT (1980) An immunoperoxidase study of neuritic plaque. J Neuropathol Exp Neurol 39:385
Puchtler H, Sweat F, Levine M (1962) On the binding of Congo red by amyloid J Histochem Cytochem 10:355–364
Schorlemmer HU, Ferluga J, Allison AC (1977) Interactions of macrophages and complement components in the pathogenesis of chronic inflammation. In: Willoughby D, Giroud JP, Velo GP (eds) Perspectives in inflammation. MTP Press, Lancaster, pp 191–206
Schwartz P (1970) Amyloidosis cause and manifestation of senile deterioration. Thomas, Springfield, IL
Stam FC (1965) Histochemistry of the senile involution of the brain. In: Vtn Int Congr Neuropathol Proc. Excerpta Medica, Amsterdam, pp 513–517
Terry RD, Wisniewski HM (1970) The ultrastructure of the neurofibrillary tangle and the senile plaque. In: Wolstenholme GEW, O'Connor M (eds) Alzheimer's disease and related conditions. Churchill, London, pp 145–180
Torack RM (1978) The pathological physiology of dementia. Springer, Berlin Heidelberg New York
Wisniewski HM, Terry RD (1973) Reexamination of the pathogenesis of the senile plaque. In: Zimmerman HM (ed) Progress in neuropathology, vol II. Grune and Stratton, New York, pp 1–26
Wigboldus JM (1959) Over de aard en genese van de plaques seniles. Thesis. Dijkstra's Drukkerij N.V., Groningen
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eikelenboom, P., Stam, F.C. Immunoglobulins and complement factors in senile plaques. Acta Neuropathol 57, 239–242 (1982). https://doi.org/10.1007/BF00685397
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00685397