Summary
One of the enigmas in the pathogenesis of inflammation is why the white cells adhere to the endothelium. In trying to define the pathogenic mechanism, we carried out experiments on ferrets infected with an SSPE strain of measles virus. Using immunoperoxidase labeling techniques, viral antigens were demonstrated on the luminal surface and in the cytoplasm of endothelial cells, irrespective of the presence or absence of inflammatory changes. The degree of inflammation corresponded well with antibody titer. These data suggest that the viral antigen in the endothelial cells is the site of interaction between these cells and sensitized lymphoid cells.
Similar content being viewed by others
References
Brown HR, Thormar H, Lin FH (1980) Immunolabeling of SSPE and wild type measles viruses in ferret brain cell culture. Acta Neuropathol (Berl) 50:181–186
Fenner F, McAuslan BR, Mims CA, Sambrook J, White DO (1974) The biology of animal viruses, 2nd edn. Academic Press, New York San Francisco London, pp 440–445
Johnson KP, Swoveland P (1977) Measles antigen distribution in brains of chronically infected hamsters. Lab Invest 37:459–465
Joseph BS, Lampert PW, Oldstone MBA (1975) Replication and persistence of measles virus in defined subpopulations of human leukocytes. J Virol 16:1638–1649
Lampert PW, Joseph BS, Oldstone MBA (1975) Antibody induced capping of measles virus antigens on plasma membrane studied by electron microscopy. J Virol 15:1248–1255
Marchesi VT, Florey HW (1960) Electron-microscopic observations on the emigration of leucocytes. Q J Exp Physiol 45:343–348
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative — a new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Mehta PD, Patrick BA, Thormar H (1982) Measles-specific IgG in CSF and serum from patients with subacute sclerosing panencephalitis. Immunology 46:423–428
Ryan US, Schultz DR, Ryan JW (1981) Fc and C3b receptors on pulmonary endothelial cells: induction by injury. Science 214:557–558
Simon GT (1965) Ultrastructure des capillaires. Symp Int Morphol Histochem Paroi Vasc, Fribourg 1965, part 1. Angiologica 2:370–434
Thormar H, Arnesen K, Mehta PD (1977) Encephalitis in ferrets caused by a non-productive strain of measles virus (DR) isolated from a patient with subacute sclerosing panencephalitis. J Infect Dis 136:229–237
Thormar H, Jervis GA, Karl SC, Brown HR (1973) Passage in ferrets of encephalitogenic cell-associated measles virus isolated from brain of a patient with subacute sclerosing panencephalitis. J Infect Dis 127:678–685
Van Pottlesburgh C, Rammohan KW, McFarland HF, Dubois-Dalcq M (1979) Selective neuronal, dendritic, and postsynaptic localization of viral antigen in measles infected mice. Lab Invest 40:99–108
Wilkinson PC, Lackie JM (1979) The adhesion migration and chemotaxis of leucocytes in inflammation. In: Movat HT (ed) Current topics in pathology, vol 68. Springer, Berlin Heidelberg New York, pp 47–88
Wisniewski HM, Lassmann H, Brosnan CF, Mehta PD, Lidsky AA, Madrid RE (1982) Multiple sclerosis: Immunological and experimental aspects. In: Matthews WB, Glaser GH (eds) Recent advances in clinical neurology. Churchill Livingstone, Edinburgh London Melbourne New York, pp 95–124
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wisniewski, H.M., Brown, H.R. & Thormar, H. Pathogenesis of viral encephalitis: Demonstration of viral antigen(s) in the brain endothelium. Acta Neuropathol 60, 107–112 (1983). https://doi.org/10.1007/BF00685354
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00685354