Summary
Nerve (NCV) and motor (MNCV) conduction velocities of the rat sciatic nerve were examined between 1 and 90 days after ischemia for 1, 2, 3, 4 or 6 h. The results were compared to light and electron microscopy of the nerve.
Slight diminution in the MNCV was observed 1 day after 1–2 h ischemia, whereas 3–6 h ischemia resulted in a complete conduction block. Diminution in the NCV occurred first after ischemia for 2 h and a complete block was seen after 4 and 6 h ischemia. Reduced NCV and MNCV were seen between 4 and 18 days only in the animals subjected to ischemia of longer duration of 3–6 h. Both the NCV and MNCV were nearly normalized at the 90th day. Ischemia of 4 and 6 h resulted in denervation of some of the muscle fibers, seen as spontaneous fibrillation at the 4th and 18th day.
Electron microscopy and histometric studies showed degeneration of myelinated fibers increasingly after longer durations of ischemia; ischemia for 2 h caused a degeneration of about 5%, 3 h of about 35%, 4 h about 45%, and 6 h about 75% of the fibers. Myelinated fibers of different sizes were equally damaged. In the teased fiber preparations normal and myelin sheaths undergoing Wallerian-like degeneration was seen. Regeneration occurred, but even at the 90th day there was a tendency of the myelin/axon ratio towards values less than control values.
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References
Boman, K.: Conductivity of sensory nerve during and after ischemia. Comm. Behav. Biol., Part A1, 151–160 (1968)
Calderwood, J. M., Dickie, W. R.: Tourniquet paresis complicating tendon grafting. Hand4, 53–57 (1972)
Dyck, P. J., Thomas, P. K., Lambert, E. H.: Neuropathy due to vascular diseases. Peripheral neuropathy, vol. I, pp. 714–733. Philadelphia-London-Toronto: W. B. Saunders Co 1975
Fowler, T. J., Danta, D., Gilliatt, R. W.: Recovery of nerve conduction after a pneumatic tourniquet: Observations on the hind limb of the baboon. J. Neurol. Neurosurg. Psychiat.35, 638–647 (1972)
Fox, J. I., Kenmore, P. I.: The effect of ischemia on nerve conduction. Exp. Neurol.17, 403–419 (1967)
Greene, E. C.: Anatomy of the rat: Transactions of the American Philosophical Society, vol. 27, pp. 199–200. New York: Hafner Publishing Co. 1955
Grundfest, H.: Effects of hydrostatic pressures upon excitability, the recovery and the potential sequence of frog nerve. Cold Spr. Harb. Symp. quant. Biol.4, 179–187 (1936)
Kamp-Nielsen, V., Kardel, T.: Decremental conduction in normal human nerves subjected to ischemia? Acta physiol. scand.92, 249–262 (1974)
Korthals, J., Wiśniewski, H. M.: Peripheral nerve ischemia. Part 1. Experimental model. J. neurol. Sci.24, 65–76 (1975)
Lorente de Nó, R., Condouris, G. A.: Decremental conduction in peripheral nerve. Integration of stimuli in the neuron. Proc. nat. Acad. Sci. (Wash.)45, 592–617 (1959)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414 (1961)
Lundborg, G.: Ischemic nerve injury. Scand. J. plast. reconstruct. Surg. Suppl. 6 (1970)
Mäkitie, J., Teräväinen, H.: Histochemical studies of striated muscle after temporary ischemia in the rat. Acta neuropath. (Berl.) (in print)
Mäkitie, J., Teräväinen, H.: Ultrastructure of rat striated muscle after temporary ischemia. Acta neuropath. (Berl.) (in print)
Ochoa, J., Fowler, T. L., Gilliatt, R. W.: Anatomical changes in peripheral nerves compressed by a pneumatic tourquet. J. Anat. (Lond.)113, 433–455 (1972)
Reynolds, E. S.: The use of lead citrate at high pH as an electronopaque stain in electronmicroscopy. J. Cell Biol.17, 208–212 (1963)
Roberts, J. T.: The effect of occlusive arterial disease of the extremities on the blood supply of nerves. Experimental and clinical studies on the role of the vasa nervorum. Amer. Heart J.35, 369–392 (1948)
Ruskin, A. P., Tanyag-Jocson, A., Rogoff, J. B.: Effect of ischemia on conduction of nerve fibers of varying diameters. Arch. Phys. Med. Rehab.48, 304–310 (1967)
Sabatini, D. D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol.17, 19–58 (1963)
Sanders, R.: The tourniquet. Instrument or weapon. Hand5, 119–123 (1973)
Seneviratne, K. N., Peiris, O. A.: The effect of ischemia on the excitability of human sensory nerve. J. Neurol. Neurosurg. Psychiat.31, 338–347 (1968)
Stock, W., Bohn, H. J.: Isselhard in co-operation with B. Schierstädt: Die Restitution des Energiestoffwechsels der Skeletmuskulatur der Ratte nach langdauernder Ischämie. Res. exp. Med.159, 306–320 (1973)
Thomason, P. R., Matzke, H. A.: Effects of ischemia on the hind limb of the rat. Amer. J. phys. Med.54, 113–131 (1975)
Watson, M. L.: Staining of tissue sections for electronmicroscopy with heavy metals. J. Cell Biol.4, 475–478 (1958)
Yamamoto, E., Fisch, U.: Experimentally induced facial nerve compression in cats. Acta oto-laryng. (Stockh.)79, 390–395 (1975)
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Mäkitie, J., Teräväinen, H. Peripheral nerve injury and recovery after temporary ischemia. Acta Neuropathol 37, 55–63 (1977). https://doi.org/10.1007/BF00684541
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DOI: https://doi.org/10.1007/BF00684541