Summary
We used a polyclonal antiserum against histamine to map histaminelike immunoreactivity (HLI) in whole mounts of the segmental ganglia and stomatogastric ganglion of crayfish and lobster. Carbodiimide fixation permitted both HRP-conjugated and FITC-conjugated secondary antibodies to be used effectively to visualize HLI in these whole mounts. Two interneurons that send axons through the inferior ventricular nerve (ivn) and the stomatogastric nerve to the stomatogastric ganglion had strong HLI, both in crayfish and in lobster. These ivn interneurons were known from other evidence to be histaminergic. The neuropil of the stomatogastric ganglion in both crayfish and lobster contained brightly labeled terminals of axons that entered the ganglion from the stomatogastric nerve. No neuronal cell bodies in this ganglion had HLI. Each segmental ganglion contained at least one pair of neurons with HLI. Some neurons in the subesophageal ganglion and in each thoracic ganglion labeled very brightly. Axons of projection interneurons with strong HLI occurred in the dorsal lateral tracts of each segmental ganglion, and sent branches to the lateral neuropils and tract neuropils of each ganglion. All the labeled neurons were interneurons; no HLI was observed in peripheral nerves.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Battelle B-A, Calman BG, Andrews AW, Grieco FD, Mleziva MB, Callaway JC, Stuart AE (1991) Histamine: a putative afferent neurotransmitter inLimulus eyes. J Comp Neurol 305:527–542
Beltz BS, Eisen JJ, Flamm R, Harris_Warrick RM, Hooper S, Marder E (1984) Serotonergic innervation and modulation of the stomatogastric ganglion of three decapod crustaceans. J Exp Biol 109:35–54
Callaway JC, Stuart AE, Edwards JS (1988) Immunohistochemical localization of histamine in the photoreceptors and segmental ganglia of the barnacle,Balanus nubilus (abstract). Neurosci Abstr 14:155.4
Chaudeonneret J (1956) La systeme nerveux de la region gnathale de l'ecrevisseCambarus affinis (Say). Ann Sci Nat 18:33–61
Claiborne BJ, Selverston AI (1984a) Histamine as a neurotransmitter in the stomatogastric nervous system of the spiny lobster. J Neurosci 4:708–721
Claiborne BJ, Selverston AI (1984b) Localization of stomatogastric IV neuron cell bodies in lobster brain. J Comp Physiol [A] 154:27–32
Dando MR, Selverston AI (1972) Command fibers from the supraoesophageal ganglion to the stomatogastric ganglion inPanulirus argus. J Comp Physiol A 78:138–175
Leise EM, Hall WM, Mulloney B (1986) Functional organization of crayfish abdominal ganglia. I. The flexor systems. J Comp Neurol 253:25–45
Leise EM, Hall WM, Mulloney B (1987) Functional organization of crayfish abdominal ganglia: II. Sensory afferents and extensor motor neurons. J Comp Neurol 266:495–518
Marder E (1987) Neurotransmitters and neuromodulators. In: Selverston AI, Moulins M (eds) The crustacean stomatogastric system. Springer, Berlin Heidelberg New York, pp 263–300
McClintock TS, Ache BW (1989) Histamine directly gates a chloride channel in lobster olfactory receptor neurons. Proc Natl Acad Sci USA 86:8137–8141
Mulloney B (1987) Neural circuits. In: Selverston AI, Moulins M (eds) The crustacean stomatogastric system. Springer, Berlin Heidelberg New York, pp 57–75
Mulloney B, Hall WM (1990) GABAergic neurons in the crayfish nervous system: an immunocytochemical census of the segmental ganglia and stomatogastric system. J Comp Neurol 291:383–394
Nässel DR, Holmqvist MH, Hardie RC, Håkanson R, Sundler F (1988) Histaminelike immunoreactivity in photoreceptors of the compound eyes and ocelli of the fliesCalliphora erythrocephala andMusca domestica. Cell Tissue Res 253:639–646
Orona E, Battelle B-A, Ache BW (1990) Immunohistochemical and biochemical evidence for the putative inhibitory neurotransmitters histamine and GABA in lobster olfactory lobes. J Comp Neurol 294:633–646
Panula P, Yang H-YT, Costa E (1984) Histamine-containing neurons in the rat hypothalamus. Proc Natl Acad Sci USA 81:2572–2576
Panula P, Happola O, Airaksinen MS, Auvinen S, Virkamaki A (1988) Carbodiimide and a tissue fixative in histamine immunohistochemistry and its application in developmental neurobiology. J Histochem Cytochem 36:259–270
Sandeman DC (1967) The vascular circulation in the brain, optic lobes, and thoracic ganglia of the crab,Carcinus. Proc R Soc Lond (Biol) 168:82–90
Sigvardt KA, Mulloney B (1982a) Properties of synapses made by IVN command interneurones in the stomatogastric ganglion of the spiny lobster,Panulirus interruptus. J Exp Biol 97:153–168
Sigvardt KA, Mulloney B (1982b) Sensory alteration of motor patterns in the stomatogastric nervous system of the spiny lobster,Panulirus interruptus. J Exp Biol 97:137–152
Simmons PJ, Hardie RC (1988) Evidence that histamine is a neurotransmitter of photoreceptors in the locust ocellus. J Exp Biol 138:205–219
Skinner K (1985) The structure of the fourth abdominal ganglion of the crayfish,Procambarus clarkii. II. Synaptic neuropils. J Comp Neurol 234:182–191
Stuart AE, Callaway JC (1988) Histamine is synthesized by barnacle ocelli and affects second-order visual cells (abstract). Invest Ophthalmol Vis Sci 29:223
Thomas JB, Bastiani MJ, Bate M, Goodman CS (1984) From grasshopper toDrosophila: a common plan for neuronal development. Nature 310:203–207
Van Harreveld A (1936) A physiological solution for freshwater crustaceans. Proc Soc Exp Biol Med 34:428–432
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mulloney, B., Hall, W.M. Neurons with histaminelike immunoreactivity in the segmental and stomatogastric nervous systems of the crayfishPacifastacus leniusculus and the lobsterHomarus americanus . Cell Tissue Res. 266, 197–207 (1991). https://doi.org/10.1007/BF00678725
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00678725