Abstract
The polystyrene (P(S)), poly(styrene/acrolein) (P(SA)), and polyacrolein (P(A)) latexes, with varied fraction of polyacrolein in the surface layer (f A=0, 0.50, 0.63, 0.84, 1.00), were used for the attachment of horseradish peroxidase. Surfaces of latexes were modified by reaction with ethylenediamine. In this step the aldehyde groups from polyacrolein were blocked and the primary amino groups were introduced. The carbohydrate portion of HRP was oxidized in the reaction leading to formation of aldehyde groups. The adsorption and covalent immobilization of HRP onto the P(S), P(SA), and P(A) latexes and of the oxidized HRP (HRP-OX) onto the modified latex particles, with amino groups on the surface (P(SA)-M and P(A)-M), were investigated. The activities of parent and oxidized HRP were compared with activities of the corresponding enzymes in solution. It has been found that whereas HRP is not suitable for the covalent immobilization on P(SA) latex and loses its activity after adsorption onto P(S) latex, HRP-OX can be adsorbed onto P(S) latex and is readily immobilized covalently onto the ethylenediamine modified P(SA) and P(A) latexes, retaining much of its former enzymatic reactivity.
Similar content being viewed by others
References
Rembaum A, Yen SPS, Molday RS (1979) J Macromol Sci, Chem A 13:603
Guiot P, Couvreur P (eds) (1986) Polymeric Nanoparticles and Microspheres. CRC, Boca Raton, FL
Rembaum A, Tökes ZA (eds) (1988) Microspheres: Medical and Biological Applications, CRC Press, Boca Raton, FL
Ugelstad J, Berge A, Ellingsen T, Schmid R, Nilsen T-N, Mørk PC, Stenstad P, Hornes E, Olsvik O (1992) Prog Colloid Polym Sci 17:87
Polysciences Inc, Data Sheet No 238 (1984) with over 70 references to original papers discussing applications of latexes and microspheres in medical diagnostics
Colowick SP, Kaplan NO (eds) (1987) Immobilized Enzymes and Cells, Part C, vol 136 In: Mosbach K (series ed) Methods in Enzymology, Academic Press, Orlando
Marumoto K, Suzuta T, Noguchi H, Uchida Y (1978) Polymer 19:867
Goers JWF (1993) In: Immunochemical Techniques; Laboratory Manual, Academic Press, San Diego, CA, p 137
Marcus L, Offarim S, Margel S (1982) Biomat, Med Dev, Art Org 10:157
Rembaum A, Yen RCK, Kempner DH, Ugelstad J (1982) J Immun Meth 52:341
Margel S (1984) J Polym Sci, Polym Chem Ed 22:3521
Rembaum A, Chang M, Richards G, Li M (1984) J Polym Sci, Polym Chem Ed 22:609
Colvin M, Smolka A, Chang M, Rembaum A (1988) In:, CRC Press, Boca Raton, FL Ref. [3] p 1
Yoshida M, Asano M, Yokota T, Chosdu R, Kumakura K (1989) J Polym Sci; Part C; Polym Lett 27:437
Yoshida M, Asano M, Yokota T, Kumakura M (1990) Polymer 31:371
Gabel D, Steinberg IZ, Katchalsky E (1971) Biochemistry 10:4461
Sandwick RK, Schray KJ (1987) J Colloid Interface Sci 115:130
Pye EK, Chance B (1976) In: Mosbach K (ed) Immobilized Enzymes, vol 44 in Methods in Enzymology. Academic Press, New York, p 111
Chang M, Colvin M, Rembaum A (1986) Polym Sci Polym Letters Ed 24:603
Chen JP, Kiaei D, Hoffman AS (1993) J Biomater Sci Polymer Edn 5:167
Malmatadt HV, Hadjioannou TP (1963) Anal Chem 35:14
Greewalt TJ, Swierk EM, Steane EA (1975) J Immunol Methods 8:351
—, Rep [8] p 119
Margel S, Beitler U, Offarim M (1982) J Cell Sci 56:157
Basinska T, Slomkowski S, Delamar M (1993) J Bioact Compat Polym 8:205
Basinska T, Slomkowski S (1993) In: Guilbault G, Mascini M (eds) Uses of Immobilized Biological Compounds. Kluwer Academic Publishers, Dordrecht, Boston, London, p 453
Basinska T, Kowalczyk D, Slomkowski S, Wang FW (1994) In: Schmitz KS (ed) Macro-ion Characterization From Dilute Solutions to Complex Fluids, ACS Symp Ser 548. ACS, Washington, DC, p 449
Miksa B, Slomkowski S (1993) In: Kahovec J (ed) Macromolecules 1992. VSP International Science Publishers, p 15
Nakane PK, Kawaoi A (1974) J Histochem Cytochem 22:1084
Ngo TT, Lenhoff HM (1980) Anal Biochem 105:389
Basinska T, Slomkowski S (1991) J Biomater Sci Polymer Edn 3:115
Basinska T, Slomkowski S (1992) In: Daniels ES, Sudol ED, El-Aasser MS (eds), Polymer Latexes, Preparation, Characterization, and Applications, ACS Symp Ser 492 p 328
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) J Biochem 193:265
Bradford MM (1976) Anal Biochem 72:248
Andrade JD, Hlady V (1986) Adv Polym Sci 79:1
Norde V, Lyklema J (1991) J Biomater Sci Polymer Edn 3:183
Nakane PK, Ram JS, Pierce GB (1966) J Histochem Cytochem 14:789
Miksa B, Slomkowski S (1995) J Biomater Sci Polymer Edn 273:47
Chen JP, Kiaei D, Hoffman AS (1993) J Biomater Sci Polymer Edn 5:167
Author information
Authors and Affiliations
Additional information
This work was supported by the KBN Grant 2 0624 91 01
Rights and permissions
About this article
Cite this article
Basinska, T., Slomkowski, S. Attachment of horseradish peroxidase (HRP) onto the poly(styrene/acrolein) latexes and onto their derivatives with amino groups on the surface; activity of immobilized enzyme. Colloid Polym Sci 273, 431–438 (1995). https://doi.org/10.1007/BF00656887
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00656887