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Localisation of genes for chloroplast components in tomato plastid DNA

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Summary

The genes for the ribosomal RNAs and for 14 chloroplast proteins have been identified and located on tomato plastid DNA by hybridisation of probes from pea, wheat or spinach chloroplast DNA. These include the genes for the large subunit of RuBP carboxylase, ATP synthase subunits alpha, beta, epsilon, I and III, photosystem I reaction centre P700, photosystem II subunits 44 kDa, 51 kDa, D2 and 32 kDa and cytochromeb-f complex subunits cytochromeb-563, cytochromef and 15 kDa polypeptide. The identity of eight of these genes was confirmed by coupled transcription-translation of plasmids and immunoprecipitation by specific antibodies. The locations of the genes are similar to their positions on spinach and tobacco plastid DNA.

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  1. Andy L. Phillips

    Present address: Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, UK

Authors and Affiliations

  1. Department of Botany, University of Cambridge, CB2 3EA, Cambridge, UK

    Andy L. Phillips

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  1. Andy L. Phillips
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Phillips, A.L. Localisation of genes for chloroplast components in tomato plastid DNA. Curr Genet 10, 153–161 (1985). https://doi.org/10.1007/BF00636481

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  • DOI: https://doi.org/10.1007/BF00636481

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