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Circadian rhythm of autophagy and light responses of autophagy and disk-shedding in the rat retina

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Summary

The rhythm of autophagic degradation (AV) in visual cell inner segments shows circadian characteristics: it persists under constant conditions of continuous darkness (DD) and continuous light (LL) and can be reentrained to phase-shifts of the light-dark (LD) cycle. However, unlike the rhythm of disk-shedding and many other circadian rhythms, the rhythm of AV persists with a distinct peak even after 3 days of LL and is rapidly abolished to almost baseline levels after 1.5 days of DD, confirming our previous observations of a strong light-dependence of AV. Since the rhythms of disk-shedding and AV reveal this inverse pattern in DD and LL, different regulative mechanisms may be involved.

Light stimulation with increasing intensities at day-time and night-time evoked AV responses that increased and disk-shedding responses that decreased at higher intensities. Furthermore, both the AV and phagosome response was different according to day-time or night-time stimulation, pointing towards the possibility of a circadian phase of sensitivity to light.

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Abbreviations

AV :

autophagic degradation, autophagic vacuole, autophay

LD :

light dark cycle

DD :

constant darkness

LL :

constant light

CNS :

central nervous system

SCN :

suprachiasmatic nucleus

DA :

dopamine

ftc :

footcandle

ANOVA :

analysis of variance

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Authors and Affiliations

  1. University Eye Clinic, Rämistrasse 100, CH-8091, Zürich, Switzerland

    C. Remé, B. Aeberhard & M. Schoch

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  1. C. Remé
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  2. B. Aeberhard
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  3. M. Schoch
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Remé, C., Aeberhard, B. & Schoch, M. Circadian rhythm of autophagy and light responses of autophagy and disk-shedding in the rat retina. J. Comp. Physiol. 156, 669–677 (1985). https://doi.org/10.1007/BF00619116

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