Summary
Measurements of local microflow are made using a small probe of about 100 mg weight with a contact surface of about 1 cm2. The probe consists of two platinum wires sealed in fused glass and the corresponding reference electrodes. One of the platinum wires is used to generate, and the other to measure hydrogen. The probe allows a qualitative continuous registration of microflow. When a simple model for hydrogen transport is assumed in blood perfused tissue, a quantitative evaluation of microflow is made by comparing H2 clearances with and without flow. For a single measurement of local microflow 15 sec are needed. Measurements can be repeated at intervals of about 4 min. The described method for hydrogen clearance permits the determination of local microflow within a tissue volume of about 2 mm3. The method has been tested with measurements of local blood flow in the cerebral cortex.
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Stosseck, K., Lübbers, D.W. & Cottin, N. Determination of local blood flow (microflow) by electrochemically generated hydrogen. Pflugers Arch. 348, 225–238 (1974). https://doi.org/10.1007/BF00587413
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DOI: https://doi.org/10.1007/BF00587413