Summary
Occupational exposure ton-hexane in shoe factory workers was monitored by measuring urinary 2,5-hexanedione, the major metabolite of this solvent and the probable cause of peripheral neuropathy in exposed workers. Solvent pollution was monitored in the work environments of 189 employees, of whom 123 (65%) worked in Alicante, Spain, and 66 (35%) in Veneto, Italy. 2,5-Hexanedione was measured in spot urine samples collected from workers at the end of the shift. Information on working conditions was obtained from a previous study. A significant linear correlation was found between mean environmental concentration ofn-hexane and urinary concentration of 2,5-hexanedione. The variability in the correlation may have been due to the variable use of protective clothing (gloves), and to variations in exposure during the working week. In numerous workers, percutaneous absorption ofn-hexane represented as much as 50% of the total absorbed dose. Urinary concentrations of 2,5-hexanedione tended to increase during the working week. Simultaneous exposure ton-hexane and toluene tended to reduce urinary excretion of 2,5-hexanedione, whereas exposure ton-hexane and methyl ethyl ketone tended to increase excretion of the metabolite.
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References
Abbritti G, Siracusa A, Cianchetti C, Coli CA, Curradi F, Perticoni GF, De Rosa E (1976) Shoe-maker's polyneuropathy in Italy: the etiological problem. Br J Ind Med 33:92–99
About-Donia MB, Makkaway HM, Graham DG (1982) The relative neurotoxicities ofn-hexane, methyln-butyl ketone, 2,5-hexanediol, and 2,5-hexanedione following oral or intraperitoneal administration in hens. Toxicol Appl Pharmacol 63:369–389
Ahonen I, Schimberg RW (1988) 2,5-Hexanedione excretion after occupational exposure ton-hexane. Br J Ind Med 45:133–136
American Conference of Governmental Industrial Hygienists (1992) Threshold limit values and biological exposure indices for 1992–93. ACGIH, Cincinnati, Ohio
Cianchetti C, Abbitti G, Perticoni GF, Siracusa A, Curradi F (1976) Toxic polyneuropathy of shoe industry workers. A study of 122 cases. J Neurol Neurosurg Psychiatry 39:1151–1161
Deutsche Forschungsgemeinschaft (1991) Maximum concentrations at the workplace and biological tolerance values for working materials 1991. VCH, New York
DiVincenzo GD, Kaplan CJ, Dedinas J (1976) Characterization of the metabolites of methyln-butyl ketone, methyl isobutyl ketone, and methyl ethyl ketone in Guinea pig serum and their clearance. Toxicol Appl Pharmacol 36:511–522
Governa M, Calisti R, Coppa G, Tagliavento G (1987) Urinary excretion of 2,5-hexanedione and peripheral polyneuropathies in workers exposed to hexane. J Toxicol Environ Health 20:219–228
Herskowitz A, Ishii N, Schaumburg HH (1971)n-Hexane neuropathy. A syndrome occurring as a result of industrial exposure. N Engl J Med 285:82–85
Jakobson I, Wahlberg JE, Holberg B, Johansson G (1982) Uptake via the blood and elimination of 10 organic solvents following epicutaneous exposure of anaesthetized guinea pigs. Toxicol Appl Pharmacol 63:181–187
Kawai T, Yasugi T, Mizunuma K, Horiguchi S, Uchida Y, Iwami O, Iguchi H, Ikeda M (1991) Dose dependent increase in 2,5-hexanedione in the urine of workers exposed ton-hexane. Occup Environ Health 63:285–291
Mutti A, Falzoi S, Lucertini S, Arfini G, Zignani M, Lombardi S, Frachini I (1984)n-Hexane metabolism in occupationally exposed workers. Br J Ind Med 41:533–538
NIOSH (1978) Manual of analytical methods. Cincinatti, Ohio
Perbellini L, Brugnone F, Pavan I (1980) Identification of the metabolites ofn-hexane, cyclohexane, and their isomers in men's urine. Toxicol Appl Pharmacol 53:220–229
Perbellini L, Brugnone F, Faggionato G (1981) Urinary excretion of the metabolites ofn-hexane and its isomers during occupational exposure. Br J Ind Med 38:20–26
Perbellini L, Leone R, Fracaso ME, Brugnone F, Venturini MS (1982) Metaolic interaction betweenn-hexane and toluene in vivo and in vitro. Int Arch Occup Environ Health 50:351–358
Perbellini L, Bartolucci GB, Brugnone F, DeRosa E, Valentini F (1985) Il 2,5-esandione nel controllo biologico dell'esposizione professionale an-esano. Med Lav 76:35–43
Perbellini L, Marhuenda Amoros D, Cardona LLorens A, Giuliari C, Brugnone F (1990) An improved method of analysing 2,5-hexanedione in urine. Br J Ind Med 47:421–424
Perbellini L, Mozzo P, Olivato D, Brugnone F (1990) “Dynamic” biological exposure indexes forn-hexane and 2,5-hexanedione, suggested by a physiologically based pharmacokinetic model. Am Ind Hyg Assoc J 51:356–362
Saito I, Shibata E, Huang J, Hisanaga N, Ono Y, Takeuchi Y (1991) Determination of urinary 2,5-hexanedione concentration by an improved analytical method as a index of exposure ton-hexane. Br J Ind Med 48:568–574
Schaumburg HH, Spencer PS (1976) Degeneration in central and peripheral nervous system by puren-hexane: an experimental study. Brain 99:183–192
Shibata E, Huang J, Ono Y, Hisanaga N, Iwata M, Saito I, Takeuchi Y (1990) Changes in urinaryn-hexane metabolites by coexposure to various concentrations of methyl ethyl ketone and fixedn-hexane levels. Arch Toxicol 64:165–168
Sobue I, Mitsumo I, Yamamura Y, Takanagui T (1978)n-Hexane induced polyneuropathy. Int J Neurol 11:317–330
Spencer PS, Couri D, Schaumburg HH (1981)n-Hexane and methyl-n-butyl ketone. In: Spencer PS, Schaumburg HH (eds) Experimental and clinical neurotoxicology. Williams & Wilkins, Baltimore, pp 456–475
Wang JD, Chang YC, Kao KP, Huang CC, Lin CC, Yeh WY (1986) An outbreak ofn-hexane induced polyneuropathy among press proofing workers in Taipei. Am J Ind Med 10:111–118
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Cardona, A., Marhuenda, D., Martí, J. et al. Biological monitoring of occupational exposure ton-hexane by measurement of urinary 2,5-hexanedione. Int. Arch Occup Environ Heath 65, 71–74 (1993). https://doi.org/10.1007/BF00586062
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DOI: https://doi.org/10.1007/BF00586062