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Kinetische Studien derd-Glucoseresorption im proximalen Konvolut der Rattenniere

Kinetic study of the local active transport ofd-glucose in the proximal convoluted tubule of rat kidney

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Summary

The proximal convoluted tubule of rat kidney was continuously perfused with a steady state solution containing 0.5 to 2.0 mM ofd-glucose. The gradual decrease of intraluminald-glucose concentration was investigated with repeated collections of perfusate from the same tubule whereby the sequence of punctures proceeded towards the site of perfusion. The rate ofd-glucose transport per unit area decreased with decreasing intraluminald-glucose concentration. This relationship could be expressed by a two parameter system corresponding to the Michaelis-Menten equation. It was found that the local maximal transport rateV max equals 6×10−10 mol×cm−2×sec−1 andK m equals 0.6 mM.

Our data on active resorption and passive permeability ofd-glucose in the proximal convolution have been subjected to computer analysis. The sum of both components ofd-glucose transport alone as measured under the condition of zero netflux of sodium chloride and water did not match the amount of net glucose transport found for the whole kidney under free-flow-conditions.

Zusammenfassung

Proximale Konvolute von Rattennieren wurden bei fehlendem Nettofluß von Natriumionen und Wasser kontinuierlich mit Lösungen perfundiert, die eined-Glucosekonzentration zwischen 0,5 und 2,0 mmol/l enthielten. Der Abfall der intraluminalend-Glucosekonzentration entlang eines Konvolutes wurde durch Absaugen der perfundierten Lösung in abnehmender Entfernung von der Perfusionsstelle verfolgt.

Die pro innere Tubulusoberfläche und Zeit transportierted-Glucosemenge wird mit Abnahme der intraluminalen Glucosekonzentration kleiner. Dieses Verhalten läßt sich durch eine 2-parametrige Gleichung analog der Michealis-Menten-Kinetik beschreiben. Es errechnet sich eine maximale Transportrate,V max, von 6 · 10−10 mol · cm−2 · sec−1 und eine Halbsättigungskonzentration,K m, von 0,6 mmol/l.

Die so beschriebene aktive Resorption und die von uns gefundene passive Permeabilität des proximalen Konvolutes fürd-Glucose reichen, nach angestellten Computerberechnungen zu schließen, allein nicht aus, um den Nettoglucosetransport der Gesamtniere unter Freiflußbedingungen quantitativ zu beschreiben.

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Authors and Affiliations

  1. Physiologisches Institut der Freien Universität Berlin, Berlin, Deutschland

    K. Loeschke, K. Baumann & F. Papavassiliou

  2. Max-Planck-Institut für Biophysik, Kennedyallee 70, 6000, Frankfurt am Main 70

    K. Loeschke, K. Baumann & F. Papavassiliou

  3. Departerment of Pharmacolocy, University of Kentucky, College of Medicine, 40506, Lexington, Kentucky

    K. Loeschke

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  1. K. Loeschke
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Auszugsweise vorgetragen auf der Frühjahrstagung der Deutschen Physiologischen Gesellschaft in Mainz 1968 [3].

Mit Unterstützung durch die Deutsche Forschungsgemeinschaft und The National Institutes of Health, Grant-No. 1 RO1 AM 10688-01.

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Loeschke, K., Baumann, K. & Papavassiliou, F. Kinetische Studien derd-Glucoseresorption im proximalen Konvolut der Rattenniere. Pflugers Arch. 305, 139–154 (1969). https://doi.org/10.1007/BF00585841

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