Abstract
In rats, pentoses and pentitols, intravenously injected in a single dose of 100 mg/kg, induced a considerable enhancement of the available pool of 5-phosphoribosyl-1-pyrophosphate and of the rate of adenine nucleotide biosynthesis in the heart, but not in liver and kidney. De novo synthesis of adenine nucleotides not detectable in skeletal muscle of normal rats became measurable after application of ribose. The stimulatory effect of isoproterenol on myocardial adenine nucleotide biosynthesis could be further potentiated by ribose and xylitol, but not by glucose. The isoproterenol-induced decrease of cardiac adenine nucleotide concentrations could be almost completely prevented by repeated administrations of ribose. Thus, pentoses and pentitols in combination with β-receptor stimulation markedly and quite specifically enhance adenine nucleotide biosynthesis in the rat heart. The results indicate that the increase in the available pool of 5-phosphoribosyl-1-pyrophosphate is an important factor for the enhancement of cardiac adenine nucleotide biosynthesis. Moreover, the availability of 5-phosphoribosyl-1-pyrophosphate and the rate of de novo synthesis of adenine nucleotides in the heart seem to be limited by the flow through the hexose monophosphate shunt.
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References
Berg, G., Matzkies, F.: Stoffwechselwirkungen einer Kohlenhydratkombinationslösung (Glucose, Fructose, Xylitol 2∶2∶1) bei parenteraler Ernährung. Dtsch. Med. Wochenschr.101, 369–373 (1976)
Burns, A. H., Reddy, W. J.: Hexose monophosphate shunt in isolated cardiac myocytes from normal rats. Am. J. Physiol.232, E 570–573 (1977)
Fleckenstein, A.: Pathophysiologische Kausalfaktoren bei Myokardnekrose und Infarkt. Wien. Z. Inn. Med.52, 133–143 (1971)
Gerlach, E., Deuticke, B., Dreisbach, R. H.: Zum Verhalten von Nucleotiden und ihren dephosphorylierten Abbauprodukten in der Niere bei Ischämie und kurzzeitiger post-ischämischer Wiederdurchblutung. Pflügers Arch. Ges. Physiol.278, 296–315 (1963)
Gerlach, E., Marko, P., Zimmer, H.-G., Pechan, I., Trendelenburg, Chr.: Different response of adenine nucleotide synthesis de novo in kidney and brain during aerobic recovery from anoxia and ischemia. Experientia27, 876–878 (1971)
Glock, G. E., McLean, P.: Levels of enzymes of the direct oxidative pathway of carbohydrate metabolism in mammalian tissues and tumors. Biochem. J.56, 171–175 (1954)
Hauschildt, S., Watts, R. W. E.: Studies on the effect of xylitol on oxalate formation. Biochem. Pharmacol.25, 27–29 (1976)
Henderson, J. F.: Feedback inhibition of purine biosynthesis in ascites tumor cells. J. Biol. Chem.237, 2631–2635 (1962)
Henderson, J. F., Khoo, M. K. Y.: On the mechanism of the feedback inhibition of purine biosynthesis de novo in Ehrlich ascites tumor cells in vitro. J. Biol. Chem.240, 3104–3109 (1963)
Henderson, J. F., Khoo, M. K. Y.: Availability of 5-phosphoribosyl-1-pyrophosphate for ribonucleotide synthesis in Ehrlich ascites tumor cells in vitro. J. Biol. Chem.240, 2358–2362 (1965)
Horecker, B. L., Smyrniotis, P. Z., Hurwitz, J.: The role of xylulose-5-phosphate in the transketolase reaction. J. Biol. Chem.223, 1009–1019 (1956)
Hori, M., Henderson, J. F.: Kinetic studies of adenine phosphoribosyltransferase. J. Biol. Chem.241, 3404–3408 (1966)
Isselhard, W., Hinzen, D. H., Geppert, E., Mäurer, W.: Beeinflussung des post-asphyktischen Wiederaufbaues der Adeninnucleotide in Kaninchenherzen in vivo durch Substratangebot. Pflügers Arch.320, 195–209 (1970)
McCormick, D. B., Touster, O.: The conversion in vivo of xylitol to glycogen via the pentose phosphate pathway. J. Biol. Chem.229, 451–461 (1957)
Zimmer, H.-G.: Effects of β-receptor stimulation and ribose supply on myocardial adenine nucleotide metabolism. Pflügers Arch.365, R 3 (1976)
Zimmer, H.-G., Trendelenburg, Chr., Gerlach, E.: Accleration of adenine nucleotide synthesis de novo during development of cardiac hypertrophy. J. Mol. Cell. Cardiol.4, 279–282 (1972)
Zimmer, H.-G., Trendelenburg, Chr., Kammermeier, H., Gerlach, E.: De novo synthesis of myocardial adenine nucleotides in the rat: Acceleration during recovery from oxygen deficiency. Circulation Res.32, 635–642 (1973)
Zimmer, H.-G., Gerlach, E.: Effect of beta-adrenergic stimulation on myocardial adenine nucleotide metabolism. Circulation Res.35, 536–543 (1974)
Zimmer, H.-G., Gerlach, E.: Changes of myocardial adenine nucleotide and protein synthesis during development of cardiac hypertrophy. Basic Res. Cardiol.72, 241–246 (1977)
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This investigation was supported in part by the Deutsche Forschungsgemeinschaft (Zi 199/1)
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Zimmer, HG., Gerlach, E. Stimulation of myocardial adenine nucleotide biosynthesis by pentoses and pentitols. Pflugers Arch. 376, 223–227 (1978). https://doi.org/10.1007/BF00584954
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DOI: https://doi.org/10.1007/BF00584954