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Reinvestigation of the transepithelial P. D. in the proximal tubule of necturus kidney

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

Published values of transpithelial potential differences (V TE) in the proximal tubule of Necturus vary from ∼0 to ∼−15 mV. In view of this disparity, we reinvestigatedV TE. Our measuredV TE was on the average −1.0 mV in early convolutions and +0.6 mV in terminal proximal segments. In the course of this study, we considered five distinct causes of artifacts. 1) Tip and pre-tip potentials: their occurrence was minimized by using Ringer's filled microelectrodes. 2) Interstitial tip localizations: the position of the tip was ascertained by the shift in potential, resulting in response to peritubular perfusion with gluconate solutions. 3) Leaky impalements:V TE responses to gluconate, input resistance determinations and the presence of positiveV TE's rule out the leak hypothesis. 4) Zeroline shifts between pre- and postimpalement stages, and 5) SpontaneousV TE drifts in the positive direction, due to gradual passage of the tip from cell to lumen, or in the negative direction, resulting from tip contact with the lower cellular layer. All five causes of artifacts may be involved in the controversy regarding pastV TE estimates.

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References

  1. Adrian RH (1958) The effect of internal and external potassium concentration on the membrane potential of frog muscle. J Physiol (Lond.) 133:631–658

    Google Scholar 

  2. Anagnostopoulos T (1975) Anion permeation in the proximal tubule of Necturus kidney. J Membr Biol 2:365–380

    Google Scholar 

  3. Anagnostopoulos T, Planelles G (1979) Organic anion permeation at the proximal tubule of Necturus. An electrophysiological study of the peritubular membrane. Pflügers Arch 381:231–239

    Google Scholar 

  4. Anagnostopoulos T, Teulon J, Edelman A (1980) Conductive properties of the proximal tubule in Necturus kidney. J Gen Physiol 75:553–587

    Google Scholar 

  5. Anagnostopoulos T, Velu E (1974) Electrical resistance of cell membranes in Necturus kidney. Pflügers Arch 346:327–339

    Google Scholar 

  6. Bott PA (1962) Micropuncture study of renal excretion of water, K, Na and Cl in Necturus. Am J Physiol 203:662–666

    Google Scholar 

  7. Boulpaep EL (1972) Permeability changes of the proximal tubule of Necturus during saline loading. Am J Physiol 222:517–531

    Google Scholar 

  8. Boulpaep EL, Seely JF (1971) Electrophysiology of proximal and distal tubules in the autoperfused dog kidney. Am J Physiol 221:1084–1096

    Google Scholar 

  9. Boulpaep EL, Steels PS (1977) Electrophysiological study of the Necturus proximal tubule: effects of organic substrates and segmental heterogeneity. In: Anagnostopoulos T (ed) Electrophysiology of the nephron. INSERM Eds. Paris, pp 127–134

    Google Scholar 

  10. Burg MB, Orloff J (1970) Electrical potential differences across proximal convoluted tubules. Am J Physiol 219:1714–1716

    Google Scholar 

  11. Davis TL, Jackson JW, Day BE, Shoemaker RL, Rehm WS (1970) Potentials in frog cornea and microelectrode artifact. Am J Physiol 219:178–183

    Google Scholar 

  12. Edelman A, Anagnostopoulos T (1976) Transepithelial potential difference in the proximal tubule of Necturus kidney. Pflügers Arch 363:105–111

    Google Scholar 

  13. Edelman A, Bouthier M, Anagnostopoulos T (1981) Chloride distribution in the proximal convoluted tubule of necturus kidney. J Membr Biol 62:7–17

    Google Scholar 

  14. Eigler FW (1961) Short circuit measurements in proximal tubule of Necturus kidney. Am J Physiol 201:157–163

    Google Scholar 

  15. Forster J, Steels PS, Boulpaep EL (1980) Organic substrate effects on and heterogeneity of Necturus proximal tubule function. Kidney Intern 17:479–490

    Google Scholar 

  16. Frömter E (1972) Progress in microelectrode techniques for kidney tubules. Yale J Biol Med 45:414–425

    Google Scholar 

  17. Frömter E, Gessner K (1974) Free-flow potential profile along rat kidney proximal tubule. Pflügers Arch 351:69–83

    Google Scholar 

  18. Frömter E, Hegel U (1966) Transtubuläre Potentialdifferenzen an proximalen und distalen Tubuli der Rattenniere. Pflügers Arch Ges Physiol 291:107–120

    Google Scholar 

  19. Garland HO, Hopkins TC, Henderson IW, Haworth CW, Chester-Jones J (1973) The application of quantitative electron probe microanalysis to renal micropuncture studies in amphibians. Micron 4:164–176

    Google Scholar 

  20. Giebisch G (1956) Measurements of pH, chloride and inulin concentrations in proximal tubule fluid of Necturus. Am J Physiol 185:171–174

    Google Scholar 

  21. Giebisch G (1961) Measurements of electrical potential differences on single nephrons of the perfused Necturus kidney. J Gen Physiol 44:659–678

    Google Scholar 

  22. Grandchamp A, Boulpaep EL (1974) Pressure control of sodium reabsorption and intercellular backflux across proximal kidney tubule. J Clin Invest 54:69–82

    Google Scholar 

  23. Kempton RT (1973) The dimensions of the renal tubules of Necturus maculosus. J Morphol 61:51–58

    Google Scholar 

  24. Khuri R, Hajjar JJ, Agulian S, Bogharian K, Kalloghlian A, Bizri H (1972) Intracellular potassium in cells of the proximal tubule of Necturus maculosus. Pflügers Arch 338:73–80

    Google Scholar 

  25. Kimura G, Spring KR (1978) Transcellular and paracellular tracer chloride fluxes in Necturus proximal tubule. Am J Physiol 235:F617-F625

    Google Scholar 

  26. Lacaz-Vieira F, Onuchic MJ (1978) Biological and artificial ion exchanger: electrical measurements with glass microelectrodes. J Membr Biol 40:157–164

    Google Scholar 

  27. MacInness DA (1961) The principles of electrochemistry. Dover Publications, New York, p 236

    Google Scholar 

  28. Nelson DJ, Ehrenfeld J, Lindemann B (1978) Volume changes and potential artifacts of epithelial cells of frog skin following impalement with microelectrodes filled with 3 M KCl. J Membr Biol 40 (special issue):91–119

    Google Scholar 

  29. Robinson RA, Stokes RH (1959) Electrolyte solutions. Butterworths, London, p 571

    Google Scholar 

  30. Sackin H, Boulpaep EL (1981) Isolated perfused salamander proximal tubule: methods, electrophysiology, and transport. Am J Physiol 241:F39-F52

    Google Scholar 

  31. Shipp JC, Hanenson IB, Windhager EE, Schatzman HJ, Whittembury G, Yoshimura H, Solomon AK (1959) Single proximal tubules of the Necturus kidney. Methods for micropuncture and microperfusion. Am J Physiol 195:563–569

    Google Scholar 

  32. Spring KR (1973) Current-induced voltage transients in Necturus proximal tubule. J Membr Biol 13:299–322

    Google Scholar 

  33. Spring KR (1977) Electrical properties of the Necturus proximal tubule. In: Anagnostopoulos T (ed) Electrophysiology of the nephron, INSERM eds, Paris, pp. 161–182

    Google Scholar 

  34. Whittembury G, Windhager EE (1961) Electrical potential difference measurements in perfused single proximal tubules of Necturus kidney. J Gen Physiol 44:679–687

    Google Scholar 

  35. Willbrandt W (1938) Electrical potential measurements across the wall of kidney tubules of Necturus. J Cell Comp Physiol 11:425–431

    Google Scholar 

  36. Windhager EE, Giebisch G (1965) Electrophysiology of the nephron. Physiol Rev 45:214–244

    Google Scholar 

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Authors and Affiliations

  1. INSERM U. 192, Hôpital Necker-Enfants Malades, Tour Technique-6 ème Etage, 149, rue de Sèvres, F-75743, Paris Cédex 15, France

    G. Planelles, K. Moreau & T. Anagnostopoulos

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  1. G. Planelles
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  2. K. Moreau
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  3. T. Anagnostopoulos
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C.N.R.S. GRECO 24

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Planelles, G., Moreau, K. & Anagnostopoulos, T. Reinvestigation of the transepithelial P. D. in the proximal tubule of necturus kidney. Pflugers Arch. 396, 41–48 (1983). https://doi.org/10.1007/BF00584696

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  • DOI: https://doi.org/10.1007/BF00584696

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