Abstract
This paper reviews the properties and regulation of single amiloride-sensitive Na+ channels in the apical membrane, and Cl− and K+ channels in the basolateral membrane of rabbit urinary bladder. According to fluctuation analysis, there is an average of one amiloride-sensitive Na+ channel for every 40 μm2 of apical membrane. Each Na+ channel passes 0.7 pA of current under normal, short-circuit conditions. Apical channels are hydrolysed by the endogenous enzyme urokinase, which is released into the urine by the kidney. After exposure to urokinase, the Na+ channel loses its amiloride sensitivity, and eventually becomes unstable in the membrane. The selectivity and kinetic properties of single anion and K+ channels in the basolateral membrane were also studied using the patch clamp technique. The properties of these channels are discussed in terms of the regulation of transepithelial Na+ transport.
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Lewis, S.A., Hanrahan, J.W. Apical and basolateral membrane ionic channels in rabbit urinary bladder epithelium. Pflugers Arch. 405 (Suppl 1), S83–S88 (1985). https://doi.org/10.1007/BF00581785
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DOI: https://doi.org/10.1007/BF00581785