Summary
By using a modified formaldehyde gas treatment, it has been possible to improve the histochemical fluorescence method for the demonstration of 5-hydroxytryptamine. A certain improvement of the localization of catecholamines has also been obtained.
The modified histochemical procedure involves a primary treatment with a formaldehyde gas of a humidity which does not cause any noticeable diffusion. After this it is possible to retreat the tissue specimen with a formaldehyde gas of higher humidity resulting in increased yields of the final fluorescent compounds. In spite of this improvement of the formaldehyde gas technique for the demonstration of 5-hydroxytryptamine, it cannot be considered as sensitive as that for catecholamines. The reasons for this are discussed.
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Fuxe, K., Jonsson, G. A modification of the histochemical fluorescence method for the improved localization of 5-hydroxytryptamine. Histochemie 11, 161–166 (1967). https://doi.org/10.1007/BF00571721
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DOI: https://doi.org/10.1007/BF00571721