Abstract
The concentrations of four vitamins, ascorbic acid, nicotinamide, choline and thiamine were evaluated in the culture supernatant of Chinese hamster ovary (CHO) cells. The media used were α-modified Eagle's minimum essential medium (MEM-α) supplemented with 10% fetal calf serum, and a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (DME/F12), containing neither serum nor protein. The reference experiment without cells revealed instability of ascorbic acid and thiamine. Moreover, a significant amount of each vitamin decreased in the culture supernatant. The possibility of growth limitation by vitamin depletion is strongly suggested.
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Kurano, S., Kurano, N., Leist, C. et al. Utilization and stability of vitamins in serum-containing and serum-free media in CHO cell culture. Cytotechnology 4, 243–250 (1990). https://doi.org/10.1007/BF00563784
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DOI: https://doi.org/10.1007/BF00563784