Abstract
Aflatoxin-producing fungi were found in fermented foods and beverages: fermented rice (kaomak), soybean sauce (taotjo), peanut butter, soy sauce (shoyu), Thai red and white wine, and rice sugar wine. These foods were extracted directly and tested for aflatoxins by thin-layer chromatography (TLC) and high pressure liquid chromatography (HPLC).
Four strains of aflatoxin-producing fungi were isolated from peanut butter, taotjo, and shoyu. Direct extracts of 10% of the peanut butters tested and 5% of the kaomak tested contained large amounts of aflatoxins. The HPLC procedure used in this experiment utilized chloroform-ethyl acetate (3∶1)
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Hesseltine, C. W., Shotwell, O. L., Kwolek, W. F., Lillehoj, E. B., Jackson, W. K. & Bothast, R. J., 1976. Aflatoxin occurrence in 1973 corn at harvest. II. Mycological studies. Mycologia. 68: 341–353.
More on the aflatoxin-hepatoma story. British Medical J. London. June 1975: 647.
Hesseltine, C. W., 1975. Mycotoxin research in India. Mycopathologia. 58(3): 157–163.
Adhemar Purchio, 1977. Search for aflatoxin B1 and similar fluorescent compounds in Miso. Mycopathologia. 58(1): 13–17.
Takahashi, D. M., 1974. TLC determination of aflatoxin in wine. J. Assoc. Off. Anal. Chem. 57(7): 875.
Goldblatt, L. A., 1969. Food Science and Technology. A series of Monographs. Academic Press, New York and London.
Thasnakorn, P., 1976. Detection of alfatoxin in ground roast peanut. Siriraj Hosp. Gaz. 28(3): 375–382.
Punpisootchai, S., 1976. Studies of contaminated moulds and their aflatoxins production in some local foods of Chiang Mai. Mahidol University, Faculty of Graduate Studies. Thesis (M.Sc. in microbiology).
Bothast, R. J. & Fennel, D. I., 1974. A medium for rapid identification and enumeration of Aspergillus flavus and related organisms. Mycologia. 66: 365–369.
Raper, R. B. & Fennel, D. I., 1965. The Genus Aspergillus. The Williums & Wilkins Company, Baltimore.
Hara, S., Fennel, D. I. & Hesseltine, C. W., 1974. Aflatoxinproducing strains of Aspergillus flavus detected by fluorescence of agar medium under UV light. Appl. Microbiol. 27: 1118.
Hesseltine, C. W., Shotwell, O. L., Ellis, J. J. & Stubblefield, R. D., 1966. Aflatoxin formation by Aspergillus flavus. Bact. Rev. 30: 795–805.
Nesheim, S., Banes, D., Stoloff, L. & Campbell, A. D., 1964. Note on aflatoxin analysis in peanuts and peanut products. J. Assoc. Office. Agri. Chemists 47: 586.
Iongh, H., Vles, R. Q. & de Vogel, P. The occurrence and detection of aflatoxin in food. In Mycotoxin in foodstuffs, Wogan, G. N. (ed.): 235–245. M.I.T. Press, Cambridge, Massachusetts.
Gilman, J. C., 1957. A manual of soil fungi. Revised second ed. The Iowa State University Press.
Eppley, R. M., 1968. Screening method for zealenone, aflatoxin, and ochratoxin. J. Assoc. Off. Anal. Chem. 51: 74.
Liem, D. H. & Beljaars, P. R., 1970. Note on rapid determination of aflatoxins in peanuts and peanut products. J. Assoc. Offic. Agr. Chem. 53(5): 1064–1066.
Nabney, J. & Nesbitt, B. F., 1965. A spectrophotometric method in determining the aflatoxins. Analyst. 90: 155–160.
Hodden, N., Baumann, F., MacDonald, F., Munk, M., Stevenson, R., Gene, D., Zamaroni, F. & Majors, R., 1971. Basic liquid chromatography. Printed in U.S.A. Copyright 1971 by Varian Aerograph.
Gupta, S. K. & Venkitasubramanian, T. A., 1975. Production of aflatoxin on soybeans. Appl. Micobiol. 29 (6): 834–836.
Mateles, R. L. & Adye, J. C., 1965. Production of aflatoxins in submerged culture. Appl. Microbiol. 13: 208–211.
Davis, N. D., Diener, U. L. & Agnihotri, V. P., 1967. Production of aflatoxins B1 and G1 in chemically defined medium. Mycopathol. Mycol. Appl. 31: 251–256.
Dollear, F. G., 1969. Detoxification of aflatoxins in foods and feeds. In food Science and Technology. A series of Monographs: 361. New York, Academic Press.
Dollear, F. G., Mann, G. E., et al. 1968. Elimination of aflatoxins from peanut meal. J. Am. Oil Chem. Soc. 45: 862–865.
Wogan, G. N., 1966. Chemical nature and biological effects of the aflatoxins. Bact. Rev. 30 (2): 460.
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Sripathomswat, N., Thasnakorn, P. Survey of aflatoxin-producing fungi in certain fermented foods and beverages in Thailand. Mycopathologia 73, 83–88 (1981). https://doi.org/10.1007/BF00562595
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DOI: https://doi.org/10.1007/BF00562595