Abstract
Reduction of the AFs produced by Aspergillus parasiticus CECT 2681 in wheat tortillas by isothiocyanates (ITCs) from oriental and yellow mustard flours was evaluated in this study. Polyethylene plastic bags were introduced with wheat tortillas contaminated with A. parasiticus and treated with 0, 0.1, 0.5 or 0.1 g of either oriental or yellow mustard flour added with 2 ml of water. The wheat tortillas were stored at room temperature during 1 month. The quantification of the AFs produced was analyzed by liquid chromatography (LC) coupled to the mass spectrometry detection in tandem (MS/MS). Gaseous allyl isothiocyanate (AITC) from oriental mustard was more effective than p-hydroxybenzyl isothiocyanate (p-HBITC) from yellow mustard to inhibit the production of AFs. More importantly, 1 g of AITC was able to reduce >90 % of AFs B1, B2, G1 and G2. p-HBITC is less stable and volatile than AITC, leading to a much lower AFs (average of 17.7 to 45.2 %). Further studies should investigate the use of active packaging using oriental mustard flour and water to reduce the production of AFs by Aspergillus species in bakery goods.
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References
Al-Gendy AA, El-gindi OD, Hafez AS, Ateya AM (2010) Glucosinolates, volatile constituents and biological activities of Erysimum corinthium Boiss. (Brassicaceae). Food Chem 118:519–524
Azaiez I, Meca G, Manyes L, Fernández-Franzón M (2013) Antifungal activity of gaseous allyl, benzyl and phenyl isothiocyanate in vitro and their use for fumonisins reduction in bread. Food Control 32:428–434
Chacon PA, Buffo RA, Holley RA (2006a) Inhibitory effects of microencapsulated allyl isothiocyanate (AIT) against Escherichia coli O157:H7 in refrigerated, nitrogen packed, finely chopped beef. Int J Food Microbiol 107:231–237
Chacon PA, Muthukumarasamy P, Holley RA (2006b) Elimination of Escherichia coli O157:H7 from fermented dry sausages at an organoleptically acceptable level of microencapsulated allyl isothiocyanate. Appl Environ Microbiol 72:3096–3102
Ciska E, Pathak DR (2004) Glucosinolate derivatives in stored fermented cabbage. J Agric Food Chem 52:7938–7943
Delaquis PJ, Mazza G (1995) Antimicrobial properties of isothiocyanates in food preservation. Food Technol 49:73–84
Delmulle BS, De Saeger SMDG, Sibanda L, Barna-Vetro I, Van Peteghem CH (2005) Development of an immuno-based lateral flow dipstick for the rapid detection of aflatoxin B1 in pig feed. J Agric Food Chem 53:3364–3368
Ekanayake A, Kester JJ, Li JJ, Zehentbauer GN, Bunke PR, Zent JB (2006) Isoguard TM: a natural anti-microbial agent derived from white mustard seed. Acta Horticult 709:101–108
Graumann GH, Holley RA (2007) Survival of Escherichia coli O157:H7 in needle-tenderized dry cured Westphalian ham. Int J Food Microbiol 118:173–179
Hasegawa N, Matsumoto Y, Hoshino A, Iwashita K (1999) Comparison of effects of Wasabia japonica and allyl isothiocyanate on the growth of four strains of Vibrio parahaemolyticus in lean and fatty tuna meat suspensions. Int J Food Microbiol 49:27–34
Hontanaya C, Meca G, Luciano FB, Mañes J, Font G (2015) Inhibition of the aflatoxins B1, B2, G1, and G2, production by Aspergillus parasiticus in nuts using yellow and oriental mustard flours. Food Control 47:154–160
Iqbal SZ, Asi MR, Ariño A, Akram N, Zuber M (2012) Aflatoxin contamination in different fractions of rice from Pakistan and estimation of dietary intakes. Mycotoxin Res 28:175–180
Jubeen F, Bhatti IA, Khan MZ, Zahoor-Ul H, Shahid M (2012) Effect of UVC irradiation on aflatoxins in ground nut (Arachis hypogea) and tree nuts (Juglans regia, prunus duclus and pistachio Vera). J Chem Soc Pak 34:1366–1374
Kelly S, Grimm LH, Bendig C, Hempel DC, Krull R (2006) Effects of fluid dynamic induced shear stress on fungal growth and morphology. Process Biochem 41:2113–2117
Lee NA, Wang S, Allan RD, Kennedy IR (2004) A rapid aflatoxin B1 ELISA: development and validation with reduced matrix effects for peanuts, corn, pistachio, and soybeans. J Agric Food Chem 52:2746–2755
Lin CM, Preston JF, Wei CI (2000) Antibacterial mechanism of allyl isothiocyanate. J Food Prot 63:727–734
Liu S, Qiu F, Kong W, Wei J, Xiao X, Yang M (2013) Development and validation of an accurate and rapid LC-ESI-MS/MS method for the simultaneous quantification of aflatoxin B1, B2, G1 and G2 in lotus seeds. Food Control 29:156–161
Luciano FB, Holley RA (2009) Enzymatic inhibition by allyl isothiocyanate and factors affecting its antimicrobial action against Escherichia coli O157:H7. Int J Food Microbiol 131:240–245
Luciano FB, Belland J, Holley RA (2011) Microbial and chemical origins of the bactericidal activity of yellow mustard powder toward Escherichia coli O157:H7 during dry sausage ripening. Int J Food Microbiol 145:69–74
Mari M, Leoni O, Bernardi R, Neri F, Palmieri S (2008) Control of brown rot on stone fruit by synthetic and glucosinolate-derived isothiocyanates. Postharvest Biol Technol 47:61–67
Méndez-Albores A, Campos-Aguilar AZ, Moreno-Martínez E, Vázquez-Durán A (2013) Physical and chemical degradation of B-aflatoxins during the roasting and dutching of cocoa liquor. J Agric Sci Technol 15:557–567
Nadarajah D, Han JH, Holley RA (2005) Inactivation of Escherichia coli O157:H7 in package ground beef by allyl isothiocyanate. Int J Food Microbiol 99:269–279
Prestera T, Fahey JW, Holtzcaw WD, Abeygunawardana C, Kachinski JL, Talalay P (1996) Comprehensive chromatographic and spectroscopic methods for the separation and identification of intact glucosinolates. Anal Biochem 239:168–179
Shin JM, Harte B, Ryser E, Selke S (2010) Active packaging of fresh chicken breast, with allyl isothiocyanate (AITC) in combination with modified atmosphere packaging (MAP) to control the growth of pathogens. J Food Sci 75:65–71
Soher EA, Amal SH (2011) Fate of aflatoxin B1 in contaminated corn gluten during acid hydrolysis. J Sci Food Agric 91:421–427
Van Egmond HP (1995) Mycotoxins: regulations, quality assurance and reference materials. Food Addit Contam 12:321–330
Whitmore BB, Naidu AS (2000) Thiosulfinates. In: Naidu AS (ed) Natural food antimicrobial systems. CRC Press, Boca Raton, pp. 349–362
Williams JH, Phillips TD, Jolly PE, Stiles JK, Jolly CM, Aggarwal D (2004) Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. Am J Clin Nutr 80:1106–1122
Zhang D, Li P, Zhang Q, Zhang W, Huang Y, Ding X, Jiang J (2009) Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure. Anal Chim Acta 636:63–69
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This research was supported by the Ministry of Economy and Competitiveness (AGL2013-43194-P).
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Quiles, J.M., Manyes, L., Luciano, F.B. et al. Effect of the oriental and yellow mustard flours as natural preservative against aflatoxins B1, B2, G1 and G2 production in wheat tortillas. J Food Sci Technol 52, 8315–8321 (2015). https://doi.org/10.1007/s13197-015-1876-2
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DOI: https://doi.org/10.1007/s13197-015-1876-2