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Veränderungen des vegetativen Nervensystems der Parotis nach Einwirkung von Antihypertensiva (Guanacline)

Ultrastrukturelle und fluorescenzcytochemische Befunde an der Rattenparotis

Alterations of the autonomic nervous system of parotid gland after administration of antihypertensiva (Guanacline)

Ultrastructural and fluorescence cytochemical findings on the parotid gland of the rat

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Summary

The effect of Guanacline on the autonomic innervation of the parotid gland of rats was studied in 70 female Wistar rats by means of electron microscopy and a cytocytochemical method (demonstration of catecholamine fluorescence after Falck). Guanacline was given intraperitoneally in a daily dose of 50 mg/kg or. 100 mg/kg body weight. To study the possible influence of hypertony on the changes in the autonomic nervous system, observations were also made on rats with hypertony with and without administration of Guanacline for comparison. In addition, the ganglion cervicale superior was studied by the same methods. The model of the ultrastructural alterations is characterized by the following signs: destruction of the granular vesicles in the terminal axons, degeneration of the mitochondria and appearance of lysosome-like bodies both in the axons and in the cytoplasm of the Schwann cells; appearance of osmiophilic bodies in the cytoplasm of the ganglion cells with destruction of the ganglion cells (70% after 6 weeks of the experiment), and lymphocytic infiltration. The depletion of biogenic amines in the sympathetic nerve fibers is parallelled by the destruction of the granular vesicles. The changes result only from the administration of Guanacline, and are not seen in rats with hypertony if Guanacline is not administered. Guanacline is thought to cause direct cytotoxic damage to the autonomic innervation of the parotid gland. The cytotoxic effect occurs at the site f resorption of substances. The changes in the parenchyma gland represent a secondary effect on the receptor tissue.

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Authors and Affiliations

  1. Pathologisches Institut der Universität Hamburg, Germany

    K. Donath & G. Seifert

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  1. K. Donath
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  2. G. Seifert
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Herrn Prof. Dr. G. Bruns, Jena, zum 60. Geburtstag gewidmet.

Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Donath, K., Seifert, G. Veränderungen des vegetativen Nervensystems der Parotis nach Einwirkung von Antihypertensiva (Guanacline). Virchows Arch. Abt. A Path. Anat. 361, 109–120 (1973). https://doi.org/10.1007/BF00557840

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  • DOI: https://doi.org/10.1007/BF00557840

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