Abstract
The aneuploids of Chinese Spring wheat have been used to locate the genes(Ti-2) coding for a novel series of trypsin inhibitors to the long arms of the homoeologous group 5 chromosomes. Three allelic variants at the 5D locus were detected in a limited survey among wheat varieties, but no variation at the loci on either chromosome 5A or chromosome 5B was detected. Homoeoloci were found in a number of alien relatives, and in the majority of cases, these were present on the group 5 homoeologue. However, inAegilops umbellulata, theTi-U2 locus was located on a chromosome presumed to belong to homoeologous group 1. NoHordeum vulgare orH. chilense Ti-2 gene was expressed in a wheat background. This new marker will be especially useful as a screening mechanism for nullisomy of chromosome 5B in work aimed at introgression of alien chromatin into wheat.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ainsworth, C. C., Miller, T. E., and Gale, M. D. (1986). The genetic control of grain esterases in hexaploid wheat. 2. Homoeologous loci in related species.Theor. Appl. Genet. 72219.
Boisen, S., and Djurtoft, R. (1981). Trypsin inhibitor from wheat endosperm: Purification and characterization.Cereal Chem. 58460.
Boisen, S., and Djurtoft, R. (1982). Protease inhibitor from barley embryo inhibiting trypsin and trypsin-like microbial proteins. Purification and characterisation of two isoforms.J. Sci. Food Agr. 33341.
Chapman, V., and Riley, R. (1970). Homoeologous meiotic chromosome pairing inTriticum aestivum in which chromosome 5B is replaced by an alien chromosome.Nature 226376.
Chojecki, A. J. S., and Gale, M. D. (1982). Genetic control of glucose phosphate isomerase in wheat and related species.Heredity 49337.
Dover, G. A. (1972).The Genetics and Function of the Meiotic Pairing-Control Systems in the Triticinae Ph.D. thesis, University of Cambridge, Cambridge.
Galili, G., and Feldman, M. (1984). Intergenomic suppression of endosperm protein genes in common wheat.Can. J. Genet. Cytol. 26651.
Hart, G. E., Islam, A. K. M. R., and Shepherd, K. W. (1980). Use of isozymes as chromosome markers in the isolation of wheat-barley addition lines.Genet. Res. (Camb.) 36311.
Hejgaard, J., Bjørn, S. E., and Nielsen, G. (1984a). Localization to chromosomes of structural genes for the major protease inhibitors of barley grains.Theor. Appl. Genet. 68127.
Hejgaard, J., Bjørn, S. E., and Nielsen, G. (1984b). Rye chromosomes carrying structural genes for the major grain protease inhibitors.Hereditas 101257.
Hochstrasser, K., and Werle, E. (1969). Reindarstellung der Trypsininhibitoren aus Keimen von Weizen- und Roggensamen, Lokalisierung der aktiven Zentren.Z. Physiol. Chem. 350249.
Islam, A. K. M. R., and Shepherd, K. W. (1982). Wheat-barley addition lines: their use in genetic and evolutionary studies of barley. InBarley Genetics IV, Proc. 4th Int. Barley Genet. Symp. Edinburgh University Press, Edinburgh, p. 729.
Kaiser, K.-P., and Belitz, H.-D. (1971). Proteinaseninhibitoren in Lebensmitteln. IV. Vorkommen und Isolierung von Trypsin- und Chymotrypsin-inhibitoren in Kartoffeln.Chem. Microbiol. Technol. Lebensm. 11.
Koebner, R. M. D. (1985).Controlled Introgression of Alien Chromatin into Wheat. Ph.D. thesis, University of Adelaide, Adelaide.
Koebner, R. M. D. (1987). Genetic control of dipeptidase in the Triticeae.Theor. Appl. Genet. (in press).
Koebner, R. M. D., and Shepherd, K. W. (1983). Shikimate dehydrogenase—a biochemical marker for group 5 chromosomes in the Triticinae.Genet. Res. (Camb.) 41209.
Koebner, R. M. D., and Shepherd, K. W. (1987). Allosyndetic recombination between a chromosome ofAegilops umbellulata and wheat chromosomes.Heredity (in press).
Law, C. N., Payne, P. I., Worland, A. J., Miller, T. E., Harris, P. A., Snape, J. W., and Reader, S. M. (1984). Studies of genetical variation affecting grain protein type and amount in wheat. InCereal Grain Protein Improvement IAEA, Vienna, p. 279.
Lawrence, G. J., and Shepherd, K. W. (1981). Chromosomal control of genes controlling seed proteins in species related to wheat.Theor. Appl. Genet. 5925.
McFadden, E. S., and Sears, E. R. (1946). The origin ofTriticum spelta and its free-threshing hexaploid relatives.J. Hered. 3781.
McIntosh, R. A. (1983). A catalogue of gene symbols for wheat. In Sakamoto, S. (ed.),Proc. 6th Int. Wheat Genet. Symp., Kyoto, p. 1197.
Mikola, J., and Kirsi, M. (1972). Differences between endospermal and embryonal trypsin inhibitors in barley, wheat, and rye.Acta Chem. Scand. 26787.
Miller, T. E., and Reader, S. M. (1987). A guide to the homoeology of chromosomes within the Triticeae.Theor. Appl. Genet. 74214.
Miller, T. E., Reader, S. M., and Chapman, V. (1982). The addition ofHordeum chilense chromosomes to wheat. InInduced Variability in Plant Breeding. Eucarpia International Symposium Pudoc, Wageningen, p. 79.
Mistunaga, T. (1979). Isolation and characterization of trypsin inhibitors from wheat germ.J. Nutr. Sci. Vitaminol. 2543.
Neuman, P. R., and Hart, G. E. (1983). Genetic control of shikimate dehydrogenase in hexaploid wheat.Biochem. Genet. 17585.
Petrucci, T., Tomasi, M., Cantagalli, P., and Silano, V. (1974). Comparison of wheat albumin inhibitors of α-amylase and trypsin.Phytochemistry 132487.
Quinones, M. A., Larter, E. N., and Samborski, D. J. (1972). The inheritance of resistance toPuccinia recondita in hexaploid triticale.Can. J. Genet. Cytol. 14495.
Riley, R. (1966). Genotype-environmental interaction affecting chiasma frequency inTriticum aestivum.Chromosomes Today 157.
Riley, R., Chapman, V., and Miller, T. E. (1973). The determination of meiotic chromosome pairing. In Sears, E. R., and Sears, L. M. S. (eds.),Proc. 4th Int. Wheat Genet. Symp. University of Missouri, Columbia, p. 731.
Sears, E. R. (1966). Nullisomic-tetrasomic combinations in hexaploid wheat. In Riley, R., and Lewis, K. R. (eds.),Chromosome Manipulations and Plant Genetics Oliver & Boyd, Edinburgh, p. 29.
Sears, E. R. (1976). A synthetic hexaploid wheat with fragile rachis.Wheat Inf. Serv. 41–4231.
Sears, E. R., and Sears, L. M. S. (1978). The telocentric chromosomes of common wheat. In Ramanujam, S. (ed.),Proc. 5th Int. Wheat Genet. Symp. Ind. Soc. Genet. & Plant Breed., Delhi, p. 389.
Shepherd, K. W. (1973). Homoeology of wheat and alien chromosomes controlling endosperm protein phenotypes. In Shepherd, K. W., and Finlay, K. W. (eds.),Proc. 3rd Int. Wheat Genet. Symp., Aust. Acad. Sci., p. 86.
Stinissen, H. M., Peumans, W. J., Law, C. N., and Payne, P. I. (1983). Control of lectins inTriticum aestivum andAegilops umbellulata by homoeologous group 1 chromosomes.Theor. Appl. Genet. 6753.
Wolf, G., Rimpau, J., and Lelley, T. (1977). Localization of structural and regulatory genes for phosphodiesterase in wheat (Triticum aestivum).Genetics 86597.
Author information
Authors and Affiliations
Additional information
The Agricultural Genetics Company is thanked for financial support.
Rights and permissions
About this article
Cite this article
Koebner, R.M.D. Genetic control of a novel series of trypsin inhibitors in wheat and its relatives. Biochem Genet 25, 591–602 (1987). https://doi.org/10.1007/BF00554360
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00554360