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Phenotypic characterization of 3H-thymidine incorporating cells in rheumatoid arthritis synovial membrane

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Summary

Tritiated-thymidine incorporating cells in synovial tissue samples from ten patients with definite or classic rheumatoid arthritis (RA) were studied by combining two techniques. Tritiated-thymidine-labelled cells were seen in autoradiography and simultaneously the subtype of them was determined with immunoperoxidase staining using monoclonal antibodies. Tritiated-thymidine-labelled cells comprised 0.8±0.4% of all the inflammatory cells in RA synovial membrane. Of all 3H-thymidine-labelled cells 34±17% were positive for OKT8 and 19±8% for OKT4 monoclonal antibodies. OKM1-positive cells comprised 7±3% of all 3H-thymidine labelled cells, whereas only a few (3±4%) of them were positive for pan-B monoclonal antibody. This study emphasizes the importance of activated OKT8 lymphocytes in RA synovial membrane.

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Authors and Affiliations

  1. Fourth Department of Medicine, Division of Rheumatic Diseases, Helsinki University Central Hospital, Unioninkatu 38, SF-00170, Helsinki 17, Finland

    P. Nykänen, V. Bergroth, D. Nordström & Y. T. Konttinen

  2. Rheumatism Foundation Hospital, SF-18120, Heinola, Finland

    P. Raunio

Authors
  1. P. Nykänen
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  2. V. Bergroth
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  3. P. Raunio
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  4. D. Nordström
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  5. Y. T. Konttinen
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Nykänen, P., Bergroth, V., Raunio, P. et al. Phenotypic characterization of 3H-thymidine incorporating cells in rheumatoid arthritis synovial membrane. Rheumatol Int 6, 269–271 (1986). https://doi.org/10.1007/BF00541318

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  • DOI: https://doi.org/10.1007/BF00541318

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