Summary
Neopterin levels of serum and fluid were determined in 30 patients with inflammatory synovial fluids and in 30 controls with joint effusions induced by trauma or osteoarthritis. Neopterin was significantly elevated in inflammatory fluids. There was no correlation with the acute phase reactants. Neopterin levels were significantly higher in inflammatory fluids than in serum, and vice versa in non-inflammatory conditions. Our results indicate that in arthritis neopterin is produced locally in the joint by monocytes induced by T-cell activation.
Similar content being viewed by others
References
Kaufmann S (1963) The structure of phenylalanine hydroxylation cofactor. Proc Natl Acad Sci USA 50:1085–1093
Curtius HC, Hausermann M, Niederwieser A (1982) Tetra-hydrobiopterin biosynthesis. In: Wachter H, Curtius HC, Pfleiderer W (eds) Biochemical and clinical aspects of pteridines. Cancer, immunology, metabolic diseases. de Gruyter, Berlin New York, pp 27–50
Ziegler I, Hamm U, Berndt J (1983) Participation of pterins in the control of lymphocyte stimulation and lymphoblast proliferation. Cancer Res 43:5356–5359
Barford PA, Blair JA, Eggar C, Hamon C, Leeming RJ (1983) Tetrahydrobiopterin metabolism and mental disease. In: Curtius HCh, Pfleiderer W, Wachter H (eds) Biochemical and clinical aspects of pteridine, vol 2. de Gruyter, Berlin New York, pp 303–315
Dhondt JL, Farriaux JP, Leroux B, Lyon I (1983) Dihydrobiopterin-synthetase deficiency. In: Curtius HCh, Pfleiderer W, Wachter H (eds) Biochemical and clinical aspects of pteridine, vol 2. de Gruyter, Berlin New York, pp 317–324
Dondt JL, Bellahsene Z, Largilliere C, Bonneterre J, Vanhille P, Noel C, Choteau P, Farriaux JP (1983) Unconjugated pteridines in human physiological fluids. Normal and pathological profiles. In: Blair JA (ed) Chemistry and biology of pteridines. de Gruyter, Berlin New York, pp 845–860
Toshiharu N, Sawada M, Yamaguchi T, Sugimoto T, Matsuura S, Akino M, Nakazawa N, Ogawa H (1983) Radioimmunoassay for neopterin in body fluid and tissue. In: Blair JA (ed) Chemistry and biology of pteridines. de Gruyter, Berlin New York, pp 821–825
Hausen A, Fuchs D, Grünewald K, Huber H, König K, Wachter H (1981) Urinary neopterine as a marker for haematological neoplasias. Clin Chim Acta 117:297–305
Hausen A, Fuchs D, Grünewald K, Huber H, König K, Wachter H (1982) Urinary neopterine in the assessment of lymphoid and myeloid neoplasia, and neopterine levels in hemolytic anemia and benign monoclonal gammopathy. Clin Biochem 15:34–37
Wachter H, Hausen A, Grassmayr K (1979) Erhöhte Ausscheidung von Neopterin im Harn von Patienten mit malignen Tumoren und mit Viruserkrankungen. Hoppe-Seylers Z Physiol Chem 360:1957–1960
Rokos K, Rokos H, Frisius H, Hüfner M (1983) Pteridines in cancer and other diseases. In: Blair JA (ed) Chemistry and biology of pteridines. de Gruyter, Berlin New York, pp 153–157
Bichler A, Hetzel H, Marth C, Fuchs D, Hausen A, Reibnegger H, Wachter H (1982) Significance of urinary neopterin in gynecology and obstetrics: oncology, pregnancy, amniotic fluid. In: Wachter H, Curtius HC, Pfleiderer W (eds) Biochemical and clinical aspects of pteridines. Cancer immunology metabolic diseases. de Gruyter, Berlin New York, pp 141–155
Bichler A, Fuchs D, Hausen A, Hetzel H, Reibnegger G, Wachter H (1983) Measurement of urinary neopterin in normal pregnant and non-pregnant women and in women with benign and malignant genital tract neoplasms. Arch Gynecol 233:121–130
Wachter H, Fuchs D, Hausen A, Huber C, Knosp O, Reibnegger G, Spira TJ (1983) Elevated urinary neopterin levels in patients with acquired immunodeficiency syndrome (AIDS). Hoppe Seyler's Z Physiol Chem 364:1345–1346
Fuchs D, Hausen A, Knosp O, Reibnegger G, Wachter H (1983) Neopterin evaluation in patients suffering from pulmonary tuberculosis. In: Blair JA (ed) Biochemical and clinical aspects of pteridines. de Gruyter, Berlin New York, pp 281–291
Reibnegger G, Boonpucknavig V, Fuchs D, Hausen A (1984) Urinary neopterin is elevated in patients with malaria. Trans R Soc Trop Med Hyg 78:545–546
Fink M, Jehn U, Wilmanns W, Rokos H, Ziegler I (1983) Neopterin in plasma and urine and biopterin in blood fractions after bone marrow transplantation. In: Blair JA (ed) Biochemical and clinical aspects of pteridines. de Gruyter, Berlin New York, pp 223–233
Fuchs D, Hausen A, Reibnegger G, Wachter H, Huber C, Margreiter R, Spielberger M (1983) Assessment of urinary neopterin in the early diagnosis of human allograft rejection. In: Blair JA (ed) Chemistry and biology of pteridines. de Gruyter, Berlin New York, pp 885–890
Hausen A, Fuchs D, Reibnegger G, Wachter H, Egg D, Günther R (1983) Neopterin as index for activity of disease in patients with rheumatoid arthritis. In: Blair JA (ed) Biochemical and clinical aspects of pteridines, vol 2. de Gruyter, Berlin New York, pp 245–254
Fuchs D, Hausen A, Huber C, Margreiter R, Reibnegger G, Spielberger M, Wachter H (1982) Pteridinausscheidung als Marker für alloantigen-induzierte Lymphocytenproliferation. Hoppe Seyler's Z Physiol Chem 363:661–664
Huber C, Fuchs D, Hausen A, Margreiter R, Reibnegger G, Spielberger M, Wachter H (1983) Pteridines as a new marker to detect human T-cells activated by allogeneic or modified self major histocompatibility complex (MHC) determinants. J Immunol 130:1047–1050
Huber C, Niederwieser D, Schönitzer D, Fuchs D, Hausen A, Knosp O, Reibnegger G, Wachter H (1983) Pteridines in the assessment of lymphocyte activation. In: Curtius HCh, Pfleiderer W, Wachter H (eds) Biochemical and clinical aspects of pteridines. de Gruyter, Berlin New York, pp 177–183
Huber C, Batchelor JR, Fuchs D, Hausen A, Lang A, Niederwieser D, Reibnegger G, Swetly P, Troppmair J, Wachter H (1984) Immune response-associated production of neopterin. J Exp Med 160:310–316
Rokos H, Rokos K (1983) A radioimmunoassay for determination of D-erythro-neopterin. In: Blair JA (ed) Chemistry and biology of pteridines. de Gruyter, Berlin New York, pp 815–819
Mancini G, Vaermann JP, Carbonara AO, Heremans JF (1965) Immunochemical quantitations by single radial immunodiffusion. Immunochemistry 2:235–238
Sachs L (1976) Biomathematik für Mediziner. Springer, Berlin Heidelberg New York, S 165–167
Sachs L (1974) Angewandte Statistik. Springer, Berlin Heidelberg New York, S 244–246
Sachs L (1984) Statistische Methoden. Springer, Berlin Heidelberg New York Tokyo, S 81–83
Hall ND, Bacon PA (1981) Lymphocyte subpopulations and their role in rheumatic disease. In: Carson Dick W (ed) Immunological aspects of rheumatology. MTP, Lancaster, pp 9–12
Levick JR (1981) Permeability of rheumatoid and normal human synovium to specific plasma proteins. Arthritis Rheum 24:1550–1560
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Maerker-Alzer, G., Diemer, O., Strümper, R. et al. Neopterin production in inflamed knee joints: high levels in synovial fluids. Rheumatol Int 6, 151–154 (1986). https://doi.org/10.1007/BF00541281
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00541281