Abstract
The calcium content of bovine rod outer segment (ROS) suspensions was determined by flame spectrophotometry to be about 0.2 Ca2+ per molecule rhodopsin. After bleaching of rhodopsin, a release of 0.01–0.1 Ca2+ per molecule rhodopsin from ROS into the solution was observed. These figures agree with some data in the literature (Appendix).
A measured absorption increase of the Ca2+-indicator phthalein purple (10 ‡C, 562 nm, pH 9.3) occurs apparently simultaneously with the formation of metarhodopsin II in ROS. This indicates that a light induced Ca2+-release of 12 calcium ions per photoactivated rhodopsin is coupled in time with the formation of metarhodopsin II.
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This work is based upon a Ph. D. dissertation (Nöll, 1974) and was presented in part at the Jahrestagung der Deutschen Gesellschaft für Biophysik, Freiburg, Germany, October 1974
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Nöll, G., Stieve, H. & Winterhager, J. Interaction of bovine rhodopsin with calcium ions. Biophys. Struct. Mechanism 5, 43–53 (1979). https://doi.org/10.1007/BF00535772
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DOI: https://doi.org/10.1007/BF00535772