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Calcium Component of the Retinal Light Response in the Snail Lymnaea stagnalis: a Pharmacological and Ultrastructural Study

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Abstract

The study aimed to analyze the role and sources of calcium ions during the retinal light response in the freshwater pond snail Lymnaea stagnalis. We explored the effect of the following pharmacological agents modulating intracellular Ca2+ concentration on the electroretinogram (ERG) of an isolated eye: 2-aminoethyl diphenyl borate, a Ca2+ current modulator; EGTA, a Ca2+ chelator; (+)-cis-dilthiazem and Cd2+, Ca2+ channel blockers. All these agents suppressed the slow ERG wave and associated impulse activity with varying degrees of effectiveness and reversibility. It was concluded that light response of microvillar photoreceptors in L. stagnalis is accompanied by an elevation of the cytoplasmic Ca2+ concentration. Together with ultrastructural features of photoreceptors, our results suggest that during the light response calcium ions are not only released from the apical intracellular Ca2+ stores, but, at least in part, enter from the extracellular medium.

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ACKNOWLEDGMENT

The authors are grateful Prof. D. von Keyserling and Mrs. A. Agbedor (Institute of Anatomy, Medical Faculty, RWTH, Aachen) for the opportunity and assistance in carrying out electron microscopy.

Funding

M.V.S. was supported by the Baltic Federal University grant for graduate students (No. 879, 12-10-2021) and a starting grant for young scientists (program 5-100).

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Authors and Affiliations

  1. Immanuel Kant Baltic Federal University, Kaliningrad, Russia

    V. V. Zhukov & M. V. Saphonov

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  1. V. V. Zhukov
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Contributions

Conceptualization and experimental design (V.V.Zh.), data collection (V.V.Zh. and M.V.S.), data processing (V.V.Zh. and M.V.S.), writing and editing a manuscript (V.V.Zh. and M.V.S.).

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Correspondence to V. V. Zhukov.

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The authors declare that they have no conflict of interest that might be associated with the publication of this article.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2022, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2022, Vol. 58, No. 3, pp. 196–208https://doi.org/10.31857/S004445292203007X.

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Zhukov, V.V., Saphonov, M.V. Calcium Component of the Retinal Light Response in the Snail Lymnaea stagnalis: a Pharmacological and Ultrastructural Study. J Evol Biochem Phys 58, 652–665 (2022). https://doi.org/10.1134/S0022093022030036

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