Abstract
Electrophoretic patterns of soluble proteins and enzymes during development from dry seed to first leaf were studied in wheat by means of disc electrophoresis. The patterns considered were those of alcohol dehydrogenase (ADH), peroxidase, and nonspecific esterases. Multiple forms (isoenzymes) were found for all these enzymes in dry seeds. The ADH pattern did not change during development. Esterases and peroxidase patterns changed both qualitatively and quantitatively. Increase in the number of peroxidase bands was marked.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhatia, C. R., and Smith, H. H. (1966). Variation associated with an Aegilops umbellulata chromosomes segment incorporated in wheat. Nature 211 1425.
Bonner, J., and Varner, J. E. (eds.) (1965). Plant Biochemistry, Academic Press, New York, p. 866.
Chen, D., Sarid, S., and Katchalski, E. (1968). Studies on the nature of messenger RNA in germinating wheat embryos. Proc. Natl. Acad. Sci. 66 902.
Davis, B. J. (1964). Disc electrophoresis. II. Methods and application to human serum proteins. Ann. N. Y. Acad. Sci. 121 404.
Hart, G. E., and Bhatia, C. R. (1967). Acrylamide gel electrophoresis of soluble leaf proteins and enzymes from Nicotiana species. Can. J. Genet. Cytol. 9 367.
Johnson, F. M., and Denniston, C. (1964). Genetic variation of alcohol dehydrogenase in Drosophila melanogaster. Nature 204 906.
Kay, E., Shannon, L. M., and Lew, J. Y. (1967). Peroxidase isozymes from horseradish roots. II. Catalytic properties. J. Biol. Chem. 242 2470.
Klapper, M. H., and Hackett, D. P. (1965). Investigations on the multiple components of commercial horseradish peroxidase. Biochim. Biophys. Acta 96 272.
Macko, J., Honold, G. R., and Stahman, M. A. (1967). Soluble proteins and multiple enzyme forms in early growth of wheat. Phytochemistry 6 464.
Marcus, A., and Feeley, J. (1965). Protein synthesis in imbibed seeds. II. Polysome formation during imbition. J. Biol. Chem. 240 1675.
Marcus, A., Feeley, J., and Volcani, T. (1966). Protein synthesis in imbibed seeds. III. Kinetics of amino acid incorporation, ribosome activation and polysome formation. Plant Physiol. 41 1167.
Markert, C. L., and Appella, E. (1961). Physicochemical nature of isozymes. Ann. N. Y. Acad. Sci. 94 678.
Markert, C. L., and Møller, F. (1959). Multiple forms of enzymes: tissue, ontogenic and species specific patterns. Proc. Natl. Acad. Sci. 45 753.
McCune, D. C. (1961). Multiple peroxidases in corn. Ann. N.Y. Acad. Sci. 94 723.
Monroy, A., and Tyler, A. (1963). Formation of active ribosomal aggregates (polysomes) upon fertilization and development of sea urchin eggs. Arch. Biochem. Biophys. 103 431.
Ockerse, R., Siegel, B. Z., and Galston, A. G. (1966). Hormone induced repression of peroxidase isozyme in plant tissues. Science 151 452.
Ornstein, L. (1964). Disc electrophoresis. I. Background and theory. Ann. N.Y. Acad. Sci. 121 321.
Scandalios, J. G. (1964). Tissue specific isozyme variation in maize. J. Heredity 55 281.
Schwartz, D. (1962). Genetic studies on mutant enzymes in maize. III. Control of gene action in the synthesis of pH 7.5 esterases. Genetics 47 1609.
Upadhya, M. D., and Yee, J. (1968). Isoenzyme polymorphism in flowering plants. VII. Isoenzyme variation in tissues of barley seedlings. Phytochemistry 7 937.
Varner, J. E., and Ram Chandra, G. (1964).Hormonal control of enzyme synthesis in barley endosperm. Proc. Natl. Acad. Sci. 52 100.
Author information
Authors and Affiliations
Additional information
Research carried out at Brookhaven National Laboratory under the auspices of the U.S. Atomic Energy Commission.
Rights and permissions
About this article
Cite this article
Bhatia, C.R., Nilson, J.P. Isoenzyme changes accompanying germination of wheat seeds. Biochem Genet 3, 207–214 (1969). https://doi.org/10.1007/BF00521137
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00521137