Summary
The positive inotropic effect of dopamine has been studied in isolated ventricular strips of guinea-pig heart.
The concentration-inotropic response curve for dopamine was significantly shifted to the right by pretreatment with reserpine.
In preparations obtained from animals pretreated with reserpine (2.5 mg/kg, 24 h prior to the experiment) the dose-response curve was not significantly affected by haloperidol, a dopamine vascular receptor antagonist (10−6−3×10−6 M).
The inotropic effect of dopamine was antagonized by practolol (3×10−7−10−6 M), but not by phentolamine (3×10−6−10−5 M); moreover the alpha-adrenoceptor blocking drug (10−5 M) did not affect the curve for dopamine in the presence of practolol (3×10−7 M).
In preparations in which fast sodium channels were blocked by K+-rich medium, slow electrical responses (calcium-mediated action potentials) as well as contractions were induced by high concentrations of dopamine (10−4−3×10−4 M); again these responses were unaffected by phentolamine or haloperidol, but were blocked by practolol.
It was concluded that in the guinea-pig ventricular muscle dopamine induced a positive inotropic effect through both indirect and direct action, and that the latter is due to the activation of beta-adrenoceptors.
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Mugelli, A., Ledda, F., Mantelli, L. et al. Studies on the positive inotropic effect of dopamine in the guinea-pig heart. Naunyn-Schmiedeberg's Arch. Pharmacol. 301, 49–55 (1977). https://doi.org/10.1007/BF00501263
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DOI: https://doi.org/10.1007/BF00501263