Skip to main content
SpringerLink
Log in

The inhibition by β-adrenoceptor blocking agents of calcium uptake into and efflux from isolated sarcoplasmic vesicles

  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Summary

The rates of calcium uptake and the calcium dependent increase in ATPase activity of sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle are reduced in the same ratio by the β-adrenoceptor blocking agents propranolol, pronethalol and Ro-03,3528, but neither by practolol, sotalol nor oxprenolol. The total calcium accumulated was affected by none of the inhibitors.

The inhibitory potency of these compounds upon the calcium pump appears to be correlated with their membrane activity or potency as local anaesthetic agents, rather than with their β-adrenoceptor blocking potency.

Like the rate of calcium uptake the rate of calcium efflux from the sarcoplasmic vesicles in the presence of ADP, GPD and UDP together with phosphate is inhibited by propranolol and pronethalol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EGTA :

ethylene glycol-bis(β-aminoethyl)-N,N1-tetraacetic acid

ADP :

adenosine diphosphate

IDP :

inosne diphosphate

CDP :

cytidine diphosphate

GDP :

guanosine diphosphate

UDP :

uridine diphosphate

INPEA :

N-isopropyl-p-nitrophenyethanolamine

References

  • Balzer, H., Makinose, M., Hasselbach, W.: The inhibition of the sarcoplasmic calcium pump by prenylamine, reserpine, chlorpromazine and imipramine. Naunyn-Schmiedeberg's Arch. Pharmak. 260, 444–455 (1968)

    Google Scholar 

  • Barlogie, B., Hasselbach, W., Makinose, M.: Activation of calcium efflux by ADP and inorganic phosphate. FEBS Letters 12, 267–268 (1971)

    Google Scholar 

  • Brunner, H., Hedwall, P. R., Meier, M.: General concepts in the use of β-blockers: the relative roles of specific and unspecific effects. Naunyn-Schmiedebergs Arch. Pharmak. 269, 219–231 (1971)

    Google Scholar 

  • Dohadwalla, A. N., Freedberg, A. S., Vaughan Williams, E. M.: The relevance of β-receptor blockade to ouabain-induced cardiac arrhythmias. Brit. J. Pharmacol. 36, 257–267 (1969)

    Google Scholar 

  • Farmer, J. B., Levy, G. P. (1970): Differentiation of β-adrenoreceptors by the use of blocking agents. J. Pharm. Pharmacol. 22, 145–146 (1970)

    Google Scholar 

  • Fitzgerald, J. D.: Perspectives in adrenergic beta-receptor blockade. Clin. Pharmacol. Ther. 10, 292–306 (1969)

    Google Scholar 

  • Friedman, Z., Makinose, M.: Phosphorylation of skeletal muscle microsomes by acetylphosphate. FEBS Letters 11, 69–72 (1970)

    Google Scholar 

  • Hasselbach, W., Makinose, M.: Über den Mechanismus des Calciumtransportes durch die Membranen des sarcoplasmatischen Reticulums. Biochem. Z. 339, 94–111 (1963)

    Google Scholar 

  • Hasselbach, W., Seraydarian, K.: The role of sulfhydryl groups in calcium transport through the sarcoplasmic membranes of skeletal muscle. Biochem. Z. 345, 159–172 (1966)

    Google Scholar 

  • Lemmer, B., Wiethold, G., Hellenbrecht, D., Bak, I. J., Grobecker, H.: Human blood platelets as cellular models for investigation of membrane active drugs: beta-adrenergic blocking agents. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 299–313 (1972)

    Google Scholar 

  • Makinose, M., Hasselbach, W.: Der Einfluß von Oxalat auf den Calcium-Transport isolierter Vesikel des sarkoplasmatischen Reticulum. Biochem. Z. 343, 360–382 (1965)

    Google Scholar 

  • Makinose, M., Hasselbach, W.: ATP synthesis by the reverse of the sarcoplasmic calcium pump. FEBS Letters 12, 271–272 (1971)

    Google Scholar 

  • Martonosi, A.: Role of phospholipids in ATPase activity and Ca transport of fragmented sarcoplasmic reticulum. Fed. Proc. 23, 913–921 (1964)

    Google Scholar 

  • Scales, B., McIntosh, D. A. D.: Effects of propranolol and its optical isomers on the radiocalcium uptake and the adenosine triphosphatases of skeletal and cardiac sarcoplasmic reticulum fractions (SRF). J. Pharmacol. exp. Ther. 160, 261–267 (1968)

    Google Scholar 

  • Singh, B. N., Vaughan Williams, E. N.: Effects on cardiac muscle of the β-adrenoceptor blocking drugs INPEA and LB46 in relation to their local anaesthetic effect on nerve. Brit. J. Pharmacol. 43, 10–22 (1971)

    Google Scholar 

  • Temple, D. M.: The effect of β-adrenergic blocking agents on the calcium pump in isolated skeletal muscle vesicles. Proc. Austr. Physiol. Pharmacol. Soc. 3, 85–86 (1972)

    Google Scholar 

  • White, R. J., Shinebourne, E.: The interaction of sympathetic stimulation and blockade on the calcium uptake of the sarcoplasmic reticulum. Cardiovasc. Res. 3, 245–248 (1969)

    Google Scholar 

Download references

Author information

Author notes

  1. Diana M. Temple

    Present address: Department of Pharmacology, University of Sydney, Sydney, Australia

Authors and Affiliations

  1. Max-Planck-Institut für medizinische Forschung Heidelberg, Germany

    Diana M. Temple, W. Hasselbach & M. Makinose

Authors
  1. Diana M. Temple
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Hasselbach
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Makinose
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Temple, D.M., Hasselbach, W. & Makinose, M. The inhibition by β-adrenoceptor blocking agents of calcium uptake into and efflux from isolated sarcoplasmic vesicles. Naunyn-Schmiedeberg's Arch. Pharmacol. 282, 187–194 (1974). https://doi.org/10.1007/BF00499033

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00499033

Key words

Search

Navigation