Abstract
Phosphoglucomutase (PGM; EC 2.7.5.1) isozyme variants were studied in a large number of inbred lines, crosses, and races of maize (Zea mays L.). Patterns of Mendelian inheritance demonstrated for PGM isozyme variants indicated that they are encoded by nuclear genes. Two unlinked loci, Pgm1 and Pgm2, located on the long arm of chromosome 1 and the short arm of chromosome 5, respectively, specify the observed electrophoretic variation on starch gels. No intra- or interlocus hybrid bands were found, suggesting that each isozyme band consists of a single polypeptide. PGM isozymes were present in all plant parts studied and the activity specified by both loci appears to reside in the cytoplasm. In studies of 520 racial collections of maize from Latin America, a single allele at each locus predominated in most collections. Likewise, the same alleles predominated in a set of 406 inbred lines of maize from the United States and Canada.
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Cardy, B. J., Stuber, C. W., and Goodman, M. M. (1980). Techniques for starch gel electrophoresis of enzymes from maize (Zea mays L.). Institute of Statistics Mimeo Series No. 1317, North Carolina State University, Raleigh.
Goodman, M. M., and Bird, R. M. (1977). The races of maize IV. Tentative grouping of 219 Latin American races. Econ. Bot. 31204.
Goodman, M. M., and Stuber, C. W. (1980). Genetic identification of lines and crosses using isoenzyme electrophoresis. Annu. Corn Sorghum Res. Conf. Proc. 3510.
Goodman, M. M., Stuber, C. W., Newton, K., and Weissinger, H. H. (1980). Linkage relationships of 19 enzyme loci in maize. Genetics 96697.
Gottlieb, L. D. (1981). Electrophoretic evidence and plant populations. Prog. Phytochem. 71.
Grobman, A., Salhuana, W., and Sevilla, R., in collaboration with Mangelsdorf, P. C. (1961). Races of maize in Peru. Their origins, evolution and classification. Natl. Acad. Sci. Natl. Res. Coun. No. 915.
Jacobson, A. B. (1968). A procedure for isolation of proplastids from etiolated maize leaves. J. Cell Biol. 38238.
Mühlbach, H., and Schnarrenberger, C. (1978). Properties and intracellular distribution of two phosphoglucomutases from spinach leaves. Planta 14165.
Paterniani, E., and Goodman, M. M. (1977). Races of maize in Brazil and adjacent areas. CIMMYT, Mexico.
Pring, D. R., and Levings, C. S., III. (1978). Heterogeneity of maize cytoplasmic genomes among male-sterile cytoplasms. Genetics 89121.
Ramirez E., R., Timothy, D. H., Diaz B., E., and Grant, U. J., in collaboration with Calle, G. E. N., Anderson, E., and Brown, W. L. (1960). Races of maize in Bolivia. Natl. Acad. Sci Natl. Res. Coun. No. 747.
Stuber, C. W., and Goodman, M. M. (1979). Genetics and linkage of PGM. Maize Genet. Coop. News Lett. 5373.
Stuber, C. W., and Goodman, M. M. (1983). Allozyme genotypes for popular and historically important inbred lines of corn. U.S. Agr. Res. Serv. (in press).
Stuber, C. W., Goodman, M. M., and Johnson, F. M. (1977). Genetic control and racial variation of β-glucosidase isozymes in maize (Zea mays L.). Biochem. Genet. 15383.
Stuber, C. W., Moll, R. H., Goodman, M. M., Schaffer, H. E., and Weir, B. S. (1980). Allozyme frequency changes associated with selection for increased grain yield in maize (Zea mays L.). Genetics 95225.
Stuber, C. W., Goodman, M. M., and Moll, R. H. (1982). Improvement of yield and ear number resulting from selection at allozyme loci in a maize population. Crop Sci. 22737.
Tanksley, S. D. (1979). Linkage, chromosomal association, and expression of Adh-1 and Pgm-2 in tomato. Biochem. Genet. 171159.
Timothy, D. H., Peña V., B., and Ramirez E., R., in collaboration with Brown, W. L., and Anderson, E. (1961). Races of maize in Chile. Natl. Acad. Sci. Natl. Res. Coun. No. 847.
Timothy, D. H., Hathaway, W. H., Grant, U. J., Torregroza C., M., Sarria V., D., and Varela A., D. (1963). Races of maize in Ecuador. Natl. Acad. Sci. Natl. Res. Coun. No. 975.
Wellhausen, E. J., Roberts, L. M., and Hernandez, “X. E.,” in collaboration with Mangelsdorf, P. C. (1952). Races of maize in Mexico. The Bussey Institution, Harvard University, Cambridge, Mass.
Wellhausen, E. J., Fuentes O., A. and Hernandez Corzo, A., in collaboration with Mangelsdorf, P. C. (1957). Races of maize in Central America. Natl. Acad. Sci. Natl. Res. Coun. No. 511.
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This work was supported in part by NIH Research Grant GM 11546.
Paper No. 8496 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, North Carolina.
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Stuber, C.W., Goodman, M.M. Inheritance, intracellular localization, and genetic variation of phosphoglucomutase isozymes in maize (Zea mays L.). Biochem Genet 21, 667–689 (1983). https://doi.org/10.1007/BF00498915
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DOI: https://doi.org/10.1007/BF00498915