Summary
Using unfixed or cryostate sections from aldehyde fixed epididymides of adult rats γ-glutamyl naphthylamides are hydrolysed in the stereocilia of the principal cells in zone 1A-4B and glycyl-prolyl naphthylamides in those of zone 1B-4A (nomenclature of Reid and Cleland, 1957); this is not the case when other amino acid and peptide naphthylamides are employed. Adenosine triphosphate (ATP) is metabolized by the stereocilia in zone 1A, 2, 3 and 4A and adenosine monophosphate (AMP) in the stereocilia of zone 3–6. With thiamine pyrophosphate (TPP) especially the Golgi apparatus of the principal cells in zone 1B and C, 2 and 3 reacts. In addition ATP, AMP and TPP stain the stereocilia of single epithelial cells outside the zones where all stereocilia react. The AMP pattern allows a further subdivision of zone 3 into 3A and B.
Substrates for the demonstration of α- and β-d-glycosidases, non-specific esterases and alkaline phosphatases are either not hydrolyzed by the stereocilia or the rate of hydrolysis is low.
Zusammenfassung
In frischen Kryostatschnitten oder nach Aldehydfixation werden im Nebenhodengang erwachsener Ratten γ-Glutamylnaphthylamide von den Stereocilien der hauptzellen in Zone 1A-4B, Glycyl-prolylnaphthylamide in denen der Zone 1B-4A umgesetzt (Nomenklatur nach Reid und Cleland, 1957); mit anderen Aminosäure- und Peptidnaphthylamiden fällt der Nachweis negativ aus. Adenosintriphosphat (ATP) wird von den Stereocilien der Zone 1A, 2, 3 und 4A gespalten; Adenosinmonophosphat (AMP) liefert in den Zonen 3–6 eine positive Stereocilienreaktion. Thiaminpyrophosphat (TPP) hydrolysiert bevorzugt der Golgi-Apparat der Hauptzellen in Zone 1B und C sowie 2 und 3. Zusätzlich werden ATP, AMP und TPP in den Stereocilien einzelner Epithelzellen außerhalb der Zonen umgesetzt, in denen die Oberfläche aller Zellen reagiert. Mit AMP als Substrat läßt sich die Zone 3 in 2 Unterzonen aufgliedern.
Substrate zum Nachweis von α- und β-d-Glycosidasen, unspezifischen Esterasen und alkalischen Phosphatasen werden von den Stereocilien entweder nicht oder kaum angegriffen.
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Herrn Obermedizinaldirektor Dr. H. Müller, Chefarzt des Kreiskrankenhauses Kronach/Ofr. zum 60. Geburtstag gewidmet.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 105).
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Gossrau, R. Zur Verteilung der Stereocilienenzyme im Nebenhodengang von Ratten. Histochemistry 57, 145–159 (1978). https://doi.org/10.1007/BF00496678
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DOI: https://doi.org/10.1007/BF00496678