Summary
The distribution of acid phosphatase, β-n-acetylglucosaminidase, β-glucuronidase, and acid β-galactosidase was studied in mm. extensor digitorum longus, soleus, and diaphragm of rats. Using the technic of semipermeable membranes activities of these enzymes were demonstrated beside cells of the interstitial tissue in muscle fibers themselves as well. Acid phosphatase displayed the highest activity which appeared in many small dots dispersed in the fiber. The activity of acid phosphatase was about 1.2 x higher in the m. soleus than in the m. extensor digitorum longus. In the latter muscle a somewhat higher activity was often found in muscle fibers displaying a higher staining for NADH tetrazolium reductase. The activity of β-n-acetylglucosaminidase was slightly lower, that of β-glucuronidase very weak but still discernible. The activity of acid β-galactosidase was not ascertained in the majority of fibers. The ratio of activities measured in an area of the same size in cells of the interstitial tissue and in muscle fibers amounted in average to 2.6: 1 in the case of acid phosphatase, 2.5:1 in the case of β-n-acetylglucosaminidase, 5.7: 1 in the case of β-glucuronidase, and 44.3:1 in the case of acid β-galactosidase. The importance of the histochemical technic in studies concerned with acid hydrolases in striated muscle fibers in normal and pathological conditions is pointed out.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arcangeli, P., Soldato, P. del, Digiesi, V., Melani, F.: Changes in the activities of lysosomal enzymes in striated muscle following ischemia. Life Sci. 12/II, 13–23 (1973)
Canonico, P.G., Bird, J.W.C.: Lysosomes in skeletal muscle tissue. Zonal centrifugation evidence for multiple cellular source. J. Cell Biol. 45, 321–333 (1970)
Fennell, R.A., West, W.T.: Oxidative and hydrolytic enzymes of homozygous dystrophic and heterozygous muscle of the house mouse. J. Histochem. Cytochem. 11, 374–382 (1963)
Goldspink, D.F., Harris, J.B., Park, D.C., Parson, M.E., Pennington, R.J.: Quantitative enzyme studies in denervated extensor digitorum longus and soleus muscles of rats. Int. J. Biochem. 2, 427–433 (1971)
Hájek, I., Gutmann, E., Syrový, I.: Proteolytic activity in denervated and reinervated muscle. Physiol. bohemoslov. 13, 32–38 (1964)
Kar, N.C., Pearson, C.M.: Acid, neutral and alkaline cathepsines in normal and diseased human muscle. Enzyme 13, 188–196 (1972)
Lockshin, R.A.: Degeneration of the intersegmental muscles. Alterations in hemolymph during muscle degeneration. Develop. Biol. 42, 28–39 (1975)
Lockshin, R.A., Beaulton, J.: Programmed cells death. Cytochemical appearance of lysosomes when the death of the intersegmented muscles is prevented. J. Ultrastruct. Res. 46, 63–78 (1974)
Lojda, Z.: Aktuelle Probleme der Cytochemie der lysosomalen Hydrolasen. Acta morph. Acad. Sci. hung. 20, 269–293 (1972)
Lojda, Z.: Histochemical methods for acid β-galactosidase:technics for semipermeable membranes. Histochemie 37, 375–378 (1973)
Lojda, Z.: Topochemistry of β-glycosidases in aortas and coronary arteries of rats, guinea pigs and rabbits in normal conditions and after cholesterol feeding. Čs. Path. 10, 1–9 (1974)
McLaughlin, J., Abood, L.G., Bosmann, H.B.: Early elevations of glycosidase, acid phosphatase, and acid proteolytic enzyme activity in denervated skeletal muscle. Exp. Neurol. 42, 541–554 (1974)
Meijer, A.E.F.H.: Semipermeable membranes for improving the histochemical determination of enzyme activities in tissue sections I. Acid phosphatase. Histochemie 30, 31–39 (1972)
Meijer, A.E.F.H., Vloedman, A.H.T.: Semipermeable membranes for improving the histochemical determination of enzyme activities in tissue sections. II. Non-specific esterase and β-glucuronidase. Histochemie 34, 127–134 (1973)
Pearce, G.W.: Electron microscopy in the study of muscular dystrophy. Ann. N.Y. Acad. Sci. 138, 138–150 (1966)
Pearson, C.M., Kar, N.C.: Hydrolytic enzyme studies in normal and diseased human muscle. Abstr. 2nd Int. Conf. Musc. Dis. Excerpta Medica ICS 237, 64 (1971)
Pollack, M.S., Bird, J.W.C.: Distribution and particle properties of acid hydrolases in denervated muscle. Amer. J. Physiol. 215, 716–722 (1968)
Schiaffino, S., Hanzliková, V.: Studies on the effect of denervation in developping muscle. II. The lysosomal system. J. Ultrastruct. Res. 39, 1–14 (1972)
Tappel, A.L., Sawnat, P.L., Shibko, S.:Lysosomes: distribution in animals, hydrolytic capacity and other properties. In: Lysosomes (de Reuck, A.V.S., Cameron, M.P., eds.), p. 78. Boston, Mass.: Little, Brown & Co. 1963
Weinstock, I.M., Iodice, A.A.: Acid hydrolase activity in muscular dystrophy and denervation atrophy. In: Lysosomes in biology and pathology (Dingle, J.T., Fell, H.B., eds.), Vol. I., pp. 450–466. Amsterdam: North Holland Publ. Co. 1969
Zalkin, H., Tappel, A.L., Caldwell, K.A., Shibko, S., Desai, I.D., Holliday, T.A.: Increased lysosomal enzymes in muscular dystrophy of vitamin E-deficient rabbits. J. biol. Chem. 237, 2678 (1962)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lojda, Z., Gutmann, E. Histochemistry of some acid hydrolases in striated muscles of the rat. Histochemistry 49, 337–342 (1976). https://doi.org/10.1007/BF00496137
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00496137