Summary
[3H]-tryptamine is taken up by rabbit platelets through an active and staurable process which is temperture sensitive, sodium-dependent and inhibited by imipramine and non-tricyclic 5-hydroxytryptamine (5-HT) uptake blockers.
There is an excellent correlation between the Ki for the inhibition of [3H]-tryptamine and [3H]-5-HT uptake in rabbit platelets for a series of 5-HT uptake blockers. These results indicate that [3H]-tryptamine is actively transported through the membrane of blood platelets by the same carrier that transports 5-HT.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Airaksinen MM, Svensk H, Tuomisto J, Komulainen H (1980) Tetrahydro β carbolines and corresponding tryptamines: In vitro inhibition of serotonin and dopamine uptake of human blood platelets. Acta Pharmacol Toxicol 46:308–313
Born GVR, Juengjaroen K, Michal F (1972) Relative activities on uptake by human blood platelets of 5-HT and several analogues. Br J Pharmacol 44:117–139
Braestrup C, Nielsen M (1980) Multiple benzodiazepine receptors. Trends in Neurosci 3:301–303
Horn AS (1973) Structure activity relations for the inhibition of 5-HT uptake into rat hypothalamic homogenates by 5-HT and tryptamine analogues. J Neurochem 21:883–888
Jones RSG (1982) Tryptamine: a neuromodulator or neurotransmitter in mammalian brain? Prog Neurobiol 19:117–139
Kellar K, Cascio C (1982) [3H]-Tryptamine high affinity binding sites in rat brain. Eur J Pharmacol 78:475–478
Langer SZ, Raisman R (1983) Binding of [3H]-imipramine and [3H]-desipramine as biochemical tools for studies in depression. Neuropharmacology 22:407–413
Langer SZ, Raisman R, Segonzac A (1983) Tryptamine and serotonin inhibit [3H]-imipramine binding in human platelets and modify the rate of dissociation. Br J Pharmacol 80:451 P
Langer SZ, Raisman R, Tahraoui L, Scatton B, Niddam R, Lee CR, Claustre Y (1984) Substituted tetrahydro-β-carbolines are possible candidates as endogenous ligand of the [3H]-imipramine recognition site. Eur J Pharmacol 98:153–154
Raisman R, Briley MS, Bouchami F, Sechter D, Zarifian E, Langer SZ (1982) [3H]-Imipramine binding and serotonin uptake in platelets from untreated depressed patients and control volunteers. Psychopharmacology 77:332–335
Ross SB, Ask AL (1980) Structural requirements for uptake into serotonergic neurones. Acta Pharmacol Toxicol 46:270–277
Sette M, Briley M, Langer SZ (1983) Complex inhibition of [3H]-imipramine binding by serotonin and non-tricyclic serotonin uptake blockers. J Neurochem 40:622–628
Wennogle LP, Meyerson L (1983) Serotonin modulates the dissociation of [3H]-imipramine from human platelets recognition sites. Eur J Pharmacol 86:303–307
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Segonzac, A., Tateishi, T. & Langer, S.Z. Saturable uptake of [3H]-tryptamine in rabbit platelets is inhibited by 5-hydroxytryptamine uptake blockers. Naunyn-Schmiedeberg's Arch. Pharmacol. 328, 33–37 (1984). https://doi.org/10.1007/BF00496102
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00496102