Summary
The distribution of 35S-taurine in rat neonates and adults was investigated by wholy-body autoradiography. The neonates (4-day-old) were frozen in dry-ice hexane at 30 min, 1, 3 and 6 h after an intraperitoneal injection of 35S-taurine, whereas survival intervals for adult rats were 1 and 3 h. Whole-sagittal sections of the frozen rat, obtained by using a cryostat microtome were dried in situ and autoradiographed. In rat neonates and adults, 35S-taurine was mainly accumulated in the renal cortex, urine, feces, liver, eye (lens, vitreous fluid, retina), hypophysis, thymus, adrenal glands, nasal mucous membrane, salivary glands, gastric mucosa, small and large intestinal mucosa, choroid plexus, myocardium and sebaceous glands. In the rat neonate, such regions as the olfactory bulb, cerebrum, and cerebellum showed relatively high optical density.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chan-Palay V, Lin C-T, Palay SL, Yamamoto M, Wu J-Y (1982a) Taurine in mammalian cerebellum: Demonstration by autoradiography with 3H-taurine and immunocytochemistry with antibodies against the taurine-synthesizing enzyme, cystein-sulfinic acid decarboxylase. Proc Natl. Acad Sci USA 79: 2695–2699
Chan-Palay V, Palay SL, Wu J-Y (1982b) Sagittal cerebellar microbands of taurine neurons: Immunocytochemical demonstration by using antibodies against the taurine-synthesizing enzyme cystein sulfinic acid decarboxylase. Proc Natl Acad Sci USA 79: 4221–4225
Davison AN, Kaczmarck LK (1971) Taurine: A possible neurotransmitter. Nature 234: 107–108
Hosokawa Y, Yamaguchi K, Kohashi N, Kori Y, Fujii O, Ueda I (1980) Immaturity of the enzyme activity and the response to inducers of rat liver cystein dioxygenase during development. J Biochem 88: 389–394
Huxtable RJ (1981) Sources and turnover rates of taurine in nursing and weaned rat pups. J Nutr 111: 1275–1286
Huxtable RJ, Lippincott SE (1982) Diet and Biosynthesis as sources of taurine in the mouse. J Nutr 112: 1003–1010
Knopf K, Sturman JA, Armstrong M, Hayes KC (1978) Taurine: An essential nutrient for the cat. J Nutr 108: 773–778
Malloy MH, Lassin DK, Gaull GE, Heird WH (1981) Development of taurine metabolism in beagle pups: Effects of taurinefree total parenteral nutrition. Biol Neonate 40: 1–8
Parmer R, Seikh KH, Dawson WW, Toskes TT (1982) A parallel change of taurine and the ERG in the developing rat retina. Comp Biochem Physiol 72C: 109–111
Rabin B, Nicolosi RJ, Hayes KC (1976) Dietary influence on bile acid conjugation in the cat. J Nutr 106: 1241–1246
Rassin DK, Sturman JA, Gaull GE (1981) Sulfur amino acid metabolism in the developing Rhesus monkey brain: Subcellular studies of taurine, cysteinsulfinic acid, and glutamic acid decarboxylase. J Neurochem 37: 740–748
Shaw RK, Heine JD (1965) Ninhydrin positive substances present in different area of normal rat brain. J Neurochem 12: 151–155
Shimada M, Suginoshita K, Wada F, Kurimoto K, Kihara T (1970) Distribution of radioactive sulfur from 35S-L-methionine in adult mice brains in vivo and its chemical analyses. Acta Anat Nippon 45: 234–241
Shimada M, Kihara T, Watanabe M, Kurimoto K (1976) The distribution of 14C from (U-14C) glucose in mice using whole-body autoradiography. J Histochem Cytochem 24: 591–600
Shimada M (1981) Glucose uptake in mouse brain regions under hypoxic hypoxia. Neurochem Res 6: 993–1003
Shimada M, Abe S, Imahayashi T (1982a) Studies on regional blood flow of the mouse using whole-body autoradiography of 14C-iodoantipyrine. Histochemie 5: 179–190
Shimada M, Murakami TH, Doi A, Abe S, Okaichi T, Watanabe M (1982b) A morphological and histochemical study on gill primary lamellae of the teleost, Seriola quinquerradiata, exposed to sea bloom. Acta Histochem Cytochem 155: 497–507
Shimada M, Murakami TH, Imahayashi T, Ozaki H (1983) Local cerebral alterations in (14C-2) deoxyglucose uptake following memory formation. J Anat 136: 751–759
Shirley AMC, Groves AD, Hesselbo T (1974) A quantitative method for measuring radioactivity in tissues sectioned for whole-body autoradiography. The International J Appl Rad Iso 25: 381–386
Sturman JA, Rassin DK, Gaull GE (1977a) Taurine in developing rat brain transfer of (35S) taurine to pups via the milk. Pediatr Res 11: 28–33
Sturman JA, Rassin DK, Gaull GE (1977b) Taurine in developing rat brain: maternal-fetal transfer of (35S) taurine and its fate in the neonate. J Neurochem 28: 31–39
Sturman JA, Rassin DK, Gaull GE (1977c) Taurine in development. Life Sci 21: 1–22
Sturman JA, Rassin DK, Hayes KC, Gaull GE (1978) Taurine Deficiency in the Kitten: Exchange and turnover of (35S) taurine in brain, retina, and other tissues. J Nutr 108: 1462–1476
Sturman JA (1979) Taurine in developing rat brain: changes in blood-brain barrier. J Neurochem 32: 811–816
Sunnof V, Grafe P, Krumniki J, Linder M, Schindler L (1975) Inhibitory post synaptic action of taurine, GABA, and other amino acids on motorneurons of isolated spinal cord. Brain Res 100: 327–341
Thurston JH, Hauhart RE, Naccarato EF (1981) Taurine: possible role in osmotic regulation of mammalian heart. Science 214: 1373–1374
Watanabe M, Kihara T, Shimada M, Kurimoto K (1978) Preparation and stain of whole-body sections. Cell Mol Biol 23: 311–315
Warwick R, Williams PL (1973) Splanchnology. In: Warwick R, Williams PL (eds) Gray's anatomy. Longman, London New York, pp 1172–1204
Yamaguchi K, Hosokawa Y, Kohashi N, Kori Y, Sakakibara S, Ueda I (1978) Rat liver cystein dioxygenase (cystein oxidase). Further purification, characterization, and analysis of the activation and inactivation. J Biochem 83: 479–491
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shimada, M., Shimono, R., Watanabe, M. et al. Distribution of 35S-taurine in rat neonates and adults. Histochemistry 80, 225–230 (1984). https://doi.org/10.1007/BF00495770
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00495770