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Immunohistochemical demonstration of carbonic anhydrase

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Summary

Rabbits were immunized using human erythroxyte carbonic anhydrase B (HCA B) purified. by the modified methods of Armstrong et al. (1966) and Bernstein and Schraer (1972). The globulin fraction was isolated by ammonium sulphate precipitation. The anti-HCA B globulin was specific, when judged using the double diffusion technique of Ouchterlony and immunoelectrophoresis. No cross reaction with human erythrocyte carbonic anhydrase C was found, but cross reactions with erythrocyte carbonic anhydrase from rat, mouse and guinea pig were observed. Fluorescein isothiocyanate conjugated goat anti-rabbit globulin was used for the localization of HCA B in tissue sections and erythrocytes on slides.

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Authors and Affiliations

  1. Department of Anatomy, University of Oulu, Kajaanintie 52A, SF-90220, Oulu 22, Finland

    Timo Kumpulainen & L. Kalevi Korhonen

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  1. Timo Kumpulainen
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  2. L. Kalevi Korhonen
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Kumpulainen, T., Korhonen, L.K. Immunohistochemical demonstration of carbonic anhydrase. Histochemistry 58, 183–192 (1978). https://doi.org/10.1007/BF00495718

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