Summary
After stimulation of the mouse peritoneal cavity with newborn calf serum (NBCS), four types of monocyte and macrophage were distinguished on the basis of peroxidase (PO) patterns. These cell types showed heterogeneity in their binding of the lectin wheat-germ agglutinin (WGA). At 16 h after stimulation, monocytes and monocyte-derived macrophages (with PO activity in granules) had a high level of WGA binding; PO-negative macrophages showed moderate WGA binding, and resident macrophages (with PO activity in the RER and nuclear envelope) had low WGA binding. At later time-points after stimulation, each of these cell types lost WGA binding sites. This decrease was related to a process of differentiation and to a modulation, affected by environmental factors. The present results also indicated that PO-negative macrophages can give rise to resident macrophages. Whether these PO-negative cells are monocyte derived or originate otherwise needs further investigation. The fourth type of macrophage, the exudate-resident cell (wtth PO activity both in granules and in the RER and nuclear envelope), with a WGA binding pattern similar to that of monocytes and monocyte-derived macrophages, was considered not to be a resident precursor cell.
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References
Beelen RHJ, Broekhuis-Fluitsma DM, Korn C, Hoefsmit ECM (1978) Identification of exudate-resident macrophages on the basis of peroxidatic activity. J Reticuloendothel Soc 23:103–110
Bursuker I, Goldman R (1982) Distinct bone marrow precursors for mononuclear phagocytes expressing high and low 5′-nucleotidase activity. J Cell Physiol 112:237–242
Bursuker I, Goldman R (1983) On the origin of macrophage heterogeneity. A hypothesis. J Reticuloendothel Soc 33:207–220
Daems WTh (1980) Peritoneal macrophages. In: Carr I, Daems WTh (eds) The reticuloendothelial system. Vol 1: Morphology. Plenum Press, New York, pp 57–127
Daems WTh, de Bakker JM (1982) Do resident macrophages proliferate? Immunobiology 161:204–211
Daems WTh, van der Rhee HJ (1980) Peroxidase and catalase in monocytes, macrophages, epithelioid cells and giant cells of the rat. In: van Furth R (ed) Mononuclear phagocytes, functional aspects, Part I. Martinus Nijhoff, The Hague, pp 43–60
Daems WTh, Roos D, van Berkel ThJC, van der Rhee HJ (1979) The subcellular distribution and biochemical properties of peroxidase in monocytes and macrophages. In: Dingle JT, Shaw IH, Jacques P (eds) Lysosomes in applied biology and therapeutics, vol 6. Elsevier North-Holland, Amsterdam pp 463–514
De Bakker JM (1983) On the origin of peritoneal resident macrophages. An electronmicroscopical study on the heterogeneity of peritoneal macrophages. Thesis, Leiden, The Netherlands
De Water R, van't Noordende JM, Ginsel LA, Daems WTh (1981) Heterogeneity in wheat germ agglutinin binding by mouse peritoneal macrophages. Histochemistry 72:333–339
De Water R, van Blitterswijk CA, Daems WTh, Ginsel LA (1982) Heterogeneity in concanavalin A binding by mouse peritoneal macrophages. Histochemistry 74:301–307
De Water R, van't Noordende JM, Onderwater JJM, van der Meer JWM, van der Gevel JS, Ginsel LA (1984) Expression of 5′-nucleotidase activity and wheat germ agglutinin binding sites in mononuclear phagocytes from bone marrow cultures. In preparation
Fukuda M, Fukuda MN (1981) Changes in cell surface glycoproteins and carbohydrate structures during the development and differentiation of human erythroid cells. J Supramol Struct Cell Biochem 17:313–324; Differentiation and function of hematopoietic cell surfaces: 97–108
Fukuda M, Koeffler HP, Minowada J (1981) Membrane differentiation in human myeloid cells: Expression of unique profiles of cell surface glycoproteins in myeloid leukemic cell lines blocked at different stages of differentiation and maturation. Proc Natl Acad Sci USA 78:6299–6303
Ginsel LA, de Water R, Onderwater JJM, Blok J, Daems WTh (1983) Heterogeneity in 5′-nucleotidase activity of mouse peritoneal macrophages. An EM-cytochemical and biochemical study. Histochemistry 79:295–309
Kaplan G, Gaudernack G (1982) In vitro differentiation of human monocytes. Differences in monocyte phenotypes induced by cultivation on glass or on collagen. J Exp Med 156:1101–1110
Kaplan G, Olstad R (1981) Heterogeneity in surface glycoproteins of mouse peritoneal macrophages. Exp Cell Res 135:379–386
Nichols BA, Bainton DF (1975) Ultrastructure and cytochemistry of mononuclear phagocytes. In: van Furth R (ed): mononuclear phargocytes in immunity, infection and pathology, Blackwell, Oxford, pp 17–55
Nicolson GL (1976) Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over cell surface components. Biochem Biophys Acta 457:57–108
Peters BP, Ebisu S, Goldstein IJ, Flashner M (1979) Interaction of wheat germ agglutinin with sialic acid. Biochemistry 24:5505–5511
Sober AJ, Haynie M, David JR (1979) Enhanced uptake of 3H-glucosamine by macrophages stimulated by macrophage activating factor. J Immunol 122:1731–1735
Van der Meer JWM, van de Gevel JS, Diesselhoff-den Dulk MMC, Beelen RHJ, van Furth R (1980) Long-term cultures of murine bone marrow mononuclear phagocytes. In: van Furth R (ed) Mononuclear phagocytes, functional aspects, part I. Martinus Nijhoff, The Hague, pp 343–361
Van der Rhee HJ, van der Burgh-de Winter CPM, Daems WTh (1979a) The differentiation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells in subcutaneous granulomas. I Fine structure. Cell Tissue Res 197:355–378
Van der Rhee HJ, van der Burgh-de Winter CPM, Daems WTh (1979b) The differentiation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells in subcutaneous granulomas. II Peroxidatic activity. Cell Tissue Res 197:379–386
Van Furth R (1982) Current view on the mononuclear phagocyte system. Immunobiology 161:178–186
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de Water, R., van't Noordende, J.M., Daems, W.T. et al. Wheat-germ agglutinin binding in four types of mouse peritoneal macrophage. Histochemistry 80, 449–456 (1984). https://doi.org/10.1007/BF00495433
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DOI: https://doi.org/10.1007/BF00495433