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Image analysis of the histochemical demonstration of glucose-6-phosphatase activity in rat liver

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Summary

Activities of histochemically demonstrated glucose-6-phosphatase were quantified by computerized densitometry using image analysis in livers of female adult Wistar rats fed ad libitum and fasted 22 h before sacrifice. Mean optical densities along the path between small portal tracts and efferent hepatic venous branches and enzyme activities obtained from biochemical assays exhibited a strong positive correlation. The gradients of high periportal to lower perivenous glucose-6-phosphatase activities were analysed by profiles of optical density along these distances.

Mapping optical densities in an image of equidensity range provided information on the distribution pattern of hepatic glucose-6-phosphatase over an extended two-dimensional area. This visualisation of histochemical enzyme reaction based on quantitative data supports the approach of sampling across the entire protal → hepatic venous distance disregarding parenchymal zonation. Utilities provided by computer assisted image analysis will have some bearing for further adequate quantitative description of liver function and structural make up.

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  1. Anatomisches Institut, Universität zu Köln, Joseph-Stelzmann-Strasse 9, D-5000, Köln 41, Germany

    R. Hildebrand & A. Schleicher

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  1. R. Hildebrand
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  2. A. Schleicher
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Supported by a grant of the Forschungsförderung des Landes Nordrhein-Westfalen, No. 40002585

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Hildebrand, R., Schleicher, A. Image analysis of the histochemical demonstration of glucose-6-phosphatase activity in rat liver. Histochemistry 86, 181–190 (1986). https://doi.org/10.1007/BF00493385

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