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In vivo identification of muscarinic receptors on rat colonic epithelial cells binding of [3H]-quinuclidinyl benzilate

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Summary

  1. (1)

    We have demonstrated that intravenously administered [3H]-quinuclidinyl benzilate binds to rat colonic and ileal epithelial cells. The binding was prevented by pretreatment with atropine and QNB.

  2. (2)

    Binding was stereoselective in favor of dexetimide, the biologically more active optical isomer of benzetimide.

  3. (3)

    The results with intestinal epithelial cells were qualitatively the same as those obtained using heart and gut muscle as controls.

  4. (4)

    QNB inhibited pilocarpine-induced fluid accumulation in ligated gut segments.

  5. (5)

    The results support the concept that cholinergic receptors, which mediate intestinal secretion, exist on rat intestinal epithelial cell membranes.

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Author notes

  1. Thomas J. Rimele

    Present address: Department of Physiology and Biophysics, Mayo Medical School, 55901, Rochester, MN

Authors and Affiliations

  1. Division of Pharmacology, College of Pharmacy, The Ohio State University, 500 West 12th Avenue, 43210, Columbus, Ohio, USA

    Thomas J. Rimele & Timothy S. Gaginella

  2. Department of Medicine, The Ohio State University, 500 West 12th Avenue, 43210, Columbus, Ohio, USA

    Thomas J. Rimele & Timothy S. Gaginella

Authors
  1. Thomas J. Rimele
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  2. Timothy S. Gaginella
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Additional information

This work was supported by NIH grant 1 R01 AM 21932

T. J. Rimele was an AFPE Albert H. Diebold Memorial Fellow during these studies.

Dr. Gaginella is the recipient of Research Career Development Award 1 K04 AM 00471-01 from the National Institutes of Health

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Rimele, T.J., Gaginella, T.S. In vivo identification of muscarinic receptors on rat colonic epithelial cells binding of [3H]-quinuclidinyl benzilate. Naunyn-Schmiedeberg's Arch. Pharmacol. 319, 18–21 (1982). https://doi.org/10.1007/BF00491472

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  • DOI: https://doi.org/10.1007/BF00491472

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