Abstract
Peptide γCB-3 is the indicator of the presence and activity of nonallelic structural genes for the human γ-chain. An equivalent peptide has been isolated from the HbF of the marmoset (Saguinus fuscicollis), the rhesus monkey (Macaca mulatta), the orangutan (Pongo pygmaeus), and the gorilla (Gorilla gorilla gorilla). The sequence of γCB-3 from the marmoset and the rhesus monkey, although different from that of human γCB-3, gives no evidence of heterogeneity of the HbF. However, the HbF of the rhesus monkey is itself heterogeneous, and there probably is a difference in another part of the γ-chain. The γCB-3 peptide of the orangutan is definitely heterogeneous: the heterogeneity is in position 135, however, rather than in position 136 as in human γCB-3. The gorilla has the same type of heterogeneity at position 136 as does the human, but the proportions of the two types of chains might differ in the two species.
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Allen, D. W., Schroeder, W. A., and Balog, J. (1958). Observations on the chromatographic heterogeneity of normal adult and fetal human hemoglobin: A study of the effects of crystallization and chromatography on the heterogeneity and isolecuine content. J. Am. Chem. Soc. 801628.
de Jong, W. W. W. (1971). Chimpanzee foetal haemoglobin: Structure and heterogeneity of the γ chain. Biochim. Biophys. Acta 251217.
Dozy, A. M., Kleihauer, E. F., and Huisman, T. H. J. (1968). Studies on the heterogeneity of hemoglobin. XIII. Chromatography of various human and animal hemoglobin types on DEAE-Sephadex. J. Chromatog. 32723.
Efremov, G. D., Huisman, T. H. J., Smith, L. L., Wilson, J. B., Kitchens, J. L., Wrightstone, R. N., and Adams, H. R. (1969). Hemoglobin Richmond, a human hemoglobin which forms asymmetric hybrids with other hemoglobins. J. Biol. Chem. 2446105.
Huisman, T. H. J. (1972). Normal and abnormal human hemoglobins. Advan. Clin. Chem. 15149.
Huisman, T. H. J., and Dozy, A. M. (1965). Studies on the heterogeneity of hemoglobin. IX. The use of tris (hydroxymethyl) aminomethane-HCl buffers in the anion-exchange chromatography of hemoglobins. J. Chromatog. 19160.
Huisman, T. H. J., Wrightstone, R. N., Wilson, J. B., Schroeder, W. A., and Kendall, A. G. (1972). Hemoglobin Kenya, the product of fusion of the γ and β polypeptide chains. Arch. Biochem. Biophys. 153850.
Jones, R. T., and Schroeder, W. A. (1963). Chromatography of human hemoglobins. Factors influencing chromatography and differentiation of similar hemoglobins. J. Chromatog. 10421.
Matsuda, G., Maita, T., Ota, H., and Takai, H. (1970). Biochemical studies on hemoglobins and myoglobins. IV. The primary structure of the α and β polypeptide chains of adult hemoglobin of the rhesus monkey (Macaca mulatta). Internat. J. Protein Res. 299.
Nute, P. E., and Stamatoyannopoulos, G. (1971). Evidence for duplication of the haemoglobin γ-chain locus in Macaca nemestrina. Nature New Biol. 229145.
Schroeder, W. A. (1972). Degradation of peptides by the Edman method with direct identification of the phenylthiohydantoin-amino acid. Meth. Enzymol. 25B258.
Schroeder, W. A., Huisman, T. H. J., Shelton, J. R., Shelton, J. B., Kleihauer, E. F., Dozy, A. M., and Robberson, B. (1968). Evidence for multiple structural genes for the γ chain of human fetal hemoglobin. Proc. Natl. Acad. Sci. 60537.
Schroeder, W. A., Huisman, T. H. J., Brown, A. K., Uy, R., Bouver, N. G., Lerch, P. O., Shelton, J. R., Shelton, J. B., and Apell, G. (1971). Postnatal changes in the chemical heterogeneity of human fetal hemoglobin. Pediat. Res. 5493.
Schroeder, W. A., Shelton, J. R., Shelton, J. B., Apell, G., Huisman, T. H. J., and Bouver, N. G. (1972). World-wide occurrence of nonallelic genes for the γ-chain of human foetal haemoglobin in newborns. Nature New Biol. 240273.
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The work was supported in part by Research Grants HL-05168 and HL-02558 from the National Institutes of Health, U.S. Public Health Service, and by NIH-Division of Research Resources Grant FR-00165.
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Huisman, T.H.J., Schroeder, W.A., Keeling, M.E. et al. Search for nonallelic structural genes for γ-chains of fetal hemoglobin in some primates. Biochem Genet 10, 309–318 (1973). https://doi.org/10.1007/BF00485708
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DOI: https://doi.org/10.1007/BF00485708