Abstract
Allozyme frequency data from natural populations of Drosophila buzzatii were analyzed for genotype-environment relationships. Allele frequency and heterozygosity at six loci polymorphic throughout eastern Australia and a number of environmental factors (both means and variabilities) were examined by a variety of multivariate techniques. Significant genotype-environment associations were found for five of the six loci, and after correcting for geographic location significant associations remained for Est-2 and Adh-1 gene frequencies and heterozygosities and for Pgm gene frequencies. The results are discussed in relation to selection and gene flow and provide the basis for laboratory studies to disentangle confounded effects of (1) environmental means and environmental variabilities and (2) allele frequency and heterozygosity, and thus to further test for and determine the nature of any natural selection at particular allozyme loci.
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References
Barker, J. S. F. (1977). Cactus-breeding Drosophila—A system for the measurement of natural selection. In Christiansen, F. B., and Fenchel, T. (eds.), Measuring Selection in Natural Populations, Vol. 19 of Lecture Notes in Biomathematics, Springer-Verlag, Berlin, p. 403.
Barker, J. S. F., and Mulley, J. C. (1976). Isozyme variation in natural populations of Drosophila buzzatii. Evolution 30213.
Bureau of Meteorology (1956). Climate Averages, Australia. Melbourne, Australia.
Bryant, E. (1974a). On the adaptive significance of enzyme polymorphisms in relation to environmental variability. Am. Nat. 1081.
Bryant, E. (1974b). An addendum on the statistical relationship between enzyme polymorphisms and environmental variability. Am. Nat. 108698.
Carson, H. L., and Wasserman, M. (1965). A widespread chromosomal polymorphism in a widespread species, Drosophila buzzatii. Am. Nat. 99111.
Christiansen, F. B., Frydenberg, O., Hjorth, J. P., and Simonsen, V. (1976). Genetics of Zoarces populations. IX. Geographic variation at the three phosphoglucomutase loci. Hereditas 83245.
Dobzhansky, T., and Ayala, F. J. (1973). Temporal frequency changes of enzyme and chromosomal polymorphisms in natural populations of Drosophila. Proc. Natl. Acad. Sci. 70680.
Hedrick, P. W., Ginevan, M. E., and Ewing, E. P. (1976). Genetic polymorphism in heterogeneous environments. Ann. Rev. Ecol. Syst. 71.
Johnson, F. M., and Schaffer, H. E. (1973). Isozyme variability in species of the genus Drosophila. VII. Genotype-environment relationships in populations of D. melanogaster from the eastern United States. Biochem. Genet. 10149.
Johnson, F. M., Schaffer, H. E., Gillaspy, J. E., and Rockwood, E. S. (1969). Isozyme genotype-environment relationships in natural populations of the harvester ant, Pogonomyrmex barbatus, from Texas. Biochem. Genet. 3429.
Johnson, G. B. (1976). Genetic polymorphism and enzyme function. In Ayala, F. J. (ed.), Molecular Evolution, Sinauer Associates, Sunderland, Mass, p. 46.
Johnson, G. B. (1977). Characterization of electrophoretically cryptic variation in the alpine butterfly Colias meadii. Biochem. Genet. 15665.
Keig, G., and McAlpine, J. R. (1969). Austclimdata: A Magnetic Tape with Estimated Mean Weekly Climatic Data for the Australian Continent, C.S.I.R.O. Division of Land Research, Technical Memorandum 69/14, 18 pp.
Kojima, K., Smouse, P., Yang, S., Nair, P. S., and Brncic, D. (1972). Isozyme frequency patterns in Drosophila pavani associated with geographical and seasonal variables. Genetics 72721.
Langley, C. H., Ito, K., and Voelker, R. A. (1977). Linkage disequilibrium in natural populations of Drosophila melanogaster: Seasonal variation. Genetics 86447.
Levins, R. (1968). Evolution in Changing Environments, Princeton University Press, Princeton, N.J.
McKechnie, S. W., Ehrlich, P. R., and White, R. R. (1975). Population genetics of Euphydryas butterflies. I. Genetic variation and the neutrality hypothesis. Genetics 81571.
Morrison, D. F. (1967). Multivariate Statistical Methods, McGraw-Hill, New York.
Mukai, T., and Voelker, R. A. (1977). The genetic structure of natural populations of Drosophila melanogaster. XIII. Further studies on linkage disequilibrium. Genetics 86175.
Mulley, J. C., and Barker, J. S. F. (1977). The occurrence and distribution of Drosophila aldrichi in Australia. Drosophila Inform. Serv. 52151.
Nevo, E., and Bar, Z. (1976). Natural selection of genetic polymorphisms along climatic gradients. In Karlin, S., and Nevo, E. (eds.), Population Genetics and Ecology, Academic Press, New York, p. 159.
Nie, N. H., Hull, C. H., Jenkins, J. G., Steinbrenner, K., and Bent, D. H. (1975). SPSS: Statistical Package for the Social Sciences, McGraw-Hill, New York.
Pipkin, S. B., Rhodes, C., and Williams, N. (1973). Influence of temperature on Drosophila alcohol dehydrogenase polymorphism. J. Hered. 64181.
Prakash, S. (1976). Gene differences between third-chromosome inversions of Drosophila pseudoobscura. Genetics 84787.
Rockwood-Sluss, E. S., Johnson, J. S., and Heed, W. B. (1973). Allozyme genotype-environment relationships. I. Variation in natural populations of Drosophila pachea. Genetics 73135.
Schaffer, H. E., and Johnson, F. M. (1974). Isozyme allelic frequencies related to selection and gene-flow hypotheses. Genetics 77163.
Selander, R. K. (1976). Genetic variation in natural populations. In Ayala, F. J. (ed.), Molecular Evolution, Sinauer Associates, Sunderland, Mass., p. 21.
Singh, R. S., Lewontin, R. C., and Felton, A. A. (1976). Genetic heterogeneity within electrophoretic “alleles” of xanthine dehydrogenase in Drosophila pseudoobscura. Genetics 84609.
Smouse, P. E., and Kojima, K. (1972). Maximum likelihood analysis of population differences in allelic frequencies. Genetics 72709.
Taylor, C. E., and Mitton, J. B. (1974). Multivariate analysis of genetic variation. Genetics 76575.
Tomaszewski, E. K., Schaffer, H. E., and Johnson, F. M. (1973). Isozyme genotype-environment associations in natural populations of the harvester ant, Pogonomyrmex badius. Genetics 75405.
Vigue, C. L., and Johnson, F. M. (1973). Isozyme variability in species of the genus Drosophila. VI. Frequency-property-environment relationships of allelic alcohol dehydrogenases in D. melanogaster. Biochem. Genet. 9213.
Wasserman, M. (1962). Cytological studies of the repleta group of the genus Drosophila: V. The mulleri subgroup. Stud. Genet. II Univ. Texas Publ. 620585.
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This work was supported by a grant from the Australian Research Grants Committee to J. S. F. B.
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Mulley, J.C., James, J.W. & Barker, J.S.F. Allozyme genotype-environment relationships in natural populations of Drosophila buzzatii . Biochem Genet 17, 105–126 (1979). https://doi.org/10.1007/BF00484477
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DOI: https://doi.org/10.1007/BF00484477