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The LSP1-α gene is not dosage compensated in the Drosophila melanogaster species subgroup

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Abstract

The X-linked α subunit of larval serum protein 1 (LSP1-α) is shown to lack dosage compensation in six members of the melanogaster species subgroup, viz., Drosophila melanogaster, D. simulans, D. mauritiana, D. erecta, D. yakuba, and D. teissieri, by quantitative filter hybridization and by electrophoretic and autoradiographic analyses of fat body proteins. These results support the hypothesis that there is little selection pressure on the LSP1-α gene to acquire dosage compensation.

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References

  • Becker, H. J. (1959). Die puffs der speicheldrusenchromosomen von Drosophila melanogaster. Chromsoma 10654.

    Google Scholar 

  • Bodenstein, D. (1965). The postembryonic development of Drosophila. In Demerec, M. (ed.), Biology of Drosophila Hafner, New York, p. 275.

    Google Scholar 

  • Brock, H. W., and Roberts, D. B. (1980). Comparison of the larval serum proteins of Drosophila melanogaster using one- and two-dimensional peptide mapping. Eur. J. Biochem. 106129.

    Google Scholar 

  • Denhardt, D. T. (1966). A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Commun. 23641.

    Google Scholar 

  • Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227680.

    Google Scholar 

  • Lucchesi, J. C. (1977). Dosage compensation: Transcription-level regulation of X-linked genes in Drosophila. Am. Zool. 17685.

    Google Scholar 

  • Muller, H. J., League, B. B., and Offerman, C. A. (1931). Effects of dosage changes of sex-linked genes and the compensatory effect of other gene differences between male and female. Anat. Rec. (Suppl.) 51110.

    Google Scholar 

  • Rigby, P. W. J., Dieckmann, M., Rhodes, C., and Berg, P. (1977). Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase 1. J. Mol. Biol. 113237.

    Google Scholar 

  • Roberts, D. B., and Evans-Roberts, S. (1979a). The genetic and cytogenetic localization of the three structural genes coding for the major protein of Drosophila larval serum. Genetics 93663.

    Google Scholar 

  • Roberts, D. B., and Evans-Roberts, S. (1979b). The X-linked α-chain gene of Drosophila LSP-1 does not show dosage compensation. Nature 280691.

    Google Scholar 

  • Roberts, D. B., Wolfe, J., and Akam, M. E. (1977). The developmental profiles of two major haemolymph proteins from Drosophila melanogaster. J. Insect Physiol. 23871.

    Google Scholar 

  • Roehrdanz, R. L., and Lucchesi, J. C. (1977). Transcriptional control and dosage compensation in Drosophila melanogaster. Nature 269243.

    Google Scholar 

  • Searle, P. F., and Tata, J. R. (1981). Vitellogenin gene expression in male Xenopus hepatocytes during primary and secondary stimulation with estrogen in cell cultures. Cell 23741.

    Google Scholar 

  • Smith, D. F., McClelland, A., White, B. N., Addison, C. F., and Glover, D. M. (1981). The molecular cloning of a dispersed set of developmentally regulated genes which encode the major larval serum protein of Drosophila melanogaster. Cell 23441.

    Google Scholar 

  • Thomas, P. S. (1980). Hybridization of denatured RNA and DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 775201.

    Google Scholar 

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H.W.B. acknowledges a Commonwealth Scholarship. This work was supported by an SRC grant to D.B.R.

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Brock, H.W., Roberts, D.B. The LSP1-α gene is not dosage compensated in the Drosophila melanogaster species subgroup. Biochem Genet 20, 287–296 (1982). https://doi.org/10.1007/BF00484425

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  • DOI: https://doi.org/10.1007/BF00484425

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