Abstract
An electrophoretic system which gives a clear separation of human hexokinases HK 1 , HK 2 , and HK 3 is described. The distribution of the hexokinase isozymes in various human tissues, both adult and fetal, is reported. Some properties of the isozymes were investigated. HK 2 was found to be more thermolabile than HK 1 , and there was also a small but significant difference in molecular size. Unlike HK 3 , HK 1 and HK 2 are not inhibited by high glucose concentrations. Screening of red cell lysates from 800 unrelated European individuals revealed no genetic variants of HK 1 and HK 2 . However, in view of their difference in properties, it seems probable that the HK 1 and HK 2 isozymes are determined by separate gene loci.
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Rogers, P.A., Fisher, R.A. & Harris, H. An electrophoretic study of the distribution and properties of human hexokinases. Biochem Genet 13, 857–866 (1975). https://doi.org/10.1007/BF00484416
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DOI: https://doi.org/10.1007/BF00484416