Abstract
Five isozymic loci were localized in the tomato (Lycopersicon esculentum) genome by trisomic analysis. Results revealed the following locations: Aps-1 on chromosome 6, Est-1 and Prx-2 on chromosome 2, Prx-4 on chromosome 10, and Prx-7 on chromosome 3. Three genes—Aps-1, Prx-2, and Prx-4—showed an arithmetic increase in allozyme concentration in direct proportion to the increase of gene dosage in respective primary trisomics. In contrast, no increase in relative Est-1 isozyme concentration was observed for any primary trisomic type. The phenotypes of the Aps-1, Prx-2, and Est-1 genes showed a pattern of banding intensity proportional to the allelic ratio (+/+/a vs. + /a/a) in primary trisomics; zymotypes of these differential trisomic heterozygotes appeared as “converse images” of each other.
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Barber, H. N., Driscoll, C. J., Long, P. M., and Vickery, R. S. (1968). Protein genetics of wheat and homoeologous relationships of chromosomes. Nature 218450.
Carlson, P. S. (1972). Locating genetic loci with aneuploids. Mol. Gen. Genet. 114273.
Clayberg, C. D., Butler, L., Kerr, E. A., Rick, C. M., and Robinson, R. W. (1966). Third list of known genes in the tomato. J. Hered. 57189.
Fobes, J. F., and Paige, D. F. (1975). Refinements in starch gel electrophoresis for screening of horticultural materials: Application to nematode testing of Lycopersicon esculentum. Hort Science 10(321.
Hagemann, R. (1969). Somatic conversion (paramutation) at the sulfurea locus of Lycopersicon escultentum Mill. III. Studies with trisomics. Can. J. Genet. Cytol. 11346.
Hart, G. E. (1970). Evidence for triplicate genes for alcohol dehydrogenase in hexaploid wheat. Proc. Natl. Acad. Sci. 66(41136.
Hart, G. E. (1975). Glutamate oxalacetate transaminase isozymes of Triticum: Evidence for multiple systems of triplicate structural genes in hexaploid wheat. In Markert, C. L. (ed.), Isozymes, Vol. III, Academic Press, New York.
Hart, G. E., and Langston, P. J. (1977). Chromosomal location and evolution of isozyme structural genes in hexaploid wheat. Heredity 39(2263–277.
Hermsen, J. G. Th., Ramanna, M. S., and Vogel, J. (1973). The location of a recessive gene for chlorophyll deficiency in diploid Solanum tuberosum by means of trisomic analysis. Can. J. Genet. Cytol. 15807.
Khush, G. S. (1973). Cytogenetics of Aneuploids, Academic Press, New York, 301 pp.
Khush, G. S., and Rick, C. M. (1967). Studies on the linkage map of chromosome 4 of the tomato and on the transmission of induced deficiencies. Genetica 3874.
Lee, H. K., and Rowe, P. R. (1975). Genetic segregation of trisomics in Solanum chacoense. J. Hered. 66131.
Lee, H. K., and Ruhde, R. W. (1976). Genetic segregation of the deformed flower gene in trisomics of Solanum chacoense. Euphytica 25313.
Lesley, J. W. (1926). The genetics of Lycopersicon esculentum Mill. I. The trisomic inheritance of “Dwarf.” Genetics 11352.
Lesley, J. W. (1928). A cytological and genetical study of progenies of triploid tomatoes. Genetics 131.
Lesley, J. W. (1932). Trisomic types of the tomato and their relations to the genes. Genetics 17545.
Lesley, J. W. (1937). Crossing over in tomatoes trisomic for the “A” or first chromosome. Genetics 22297.
McDaniel, R. G., and Ramage, R. T. (1970). Genetics of a primary trisomic series in barley: Identification by protein electrophoresis. Can. J. Genet. Cytol. 12490.
Nielsen, G., and Frydenberg, O. (1971). Chromosome localization of the esterase loci Est-1 and Est-2 in barley by means of trisomics. Hereditas 67152.
Nielsen, G., and Scandalios, J. G. (1974). Chromosomal location by use of trisomics and new alleles of an endopeptidase in Zea mays. Genetics 77679.
Nishikawa, K. and Nobuhara, M. (1971). Genetic studies of α-amylase isozymes in wheat. I. Location and variation in tetra-and hexaploid wheat. Jpn. J. Genet. 46(5345.
Rick, C. M. (1971). Some cytogenetic features of the genome in diploid plant species. Stadler Genet. Symp. 1,2153.
Rick, C. M. (1975). The tomato. In King, R. C. (ed.), Handbook of Genetics, Vol. 2, Plenum Press, New York, 247 pp.
Rick, C. M. (1977). Linkage map. Rep. Tomato Gen. Coop. 275.
Rick, C. M., and Barton, D. W. (1954). Cytological and genetical identification of the primary trisomics of the tomato. Genetics 39640.
Rick, C. M., and Fobes, J. F. (1974). Association of an allozyme with nematode resistance. Rep. Tomato Gen. Coop. 2425.
Rick, C. M., and Fobes, J. F. (1977). Linkage relations of some isozyme loci. Rep. Tomato Gen. Coop. 2722.
Rick, C. M., Dempsey, W. H., and Khush, G. S. (1964). Further studies on the primary trisomics of the tomato. Can. J. Genet. Cytol. 693.
Rick, C. M., Fobes, J. F., and Holle, M. (1977). Genetic variation in Lycopersicon pimpinellifolium: Evidence of evolutionary change in mating systems. Plant System. Evol. 127139.
Smith, H. H., and Conklin, M. E. (1975). Effects of gene dosage on perioxidase isozyme in Datura stramonium trisomics. In Markert, C. L. (ed.), Isozymes, Vol. III, Academic Press, New York.
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This research was performed under the auspices of NSF Grants BMS75-03024 and DEB77-02248 to C. M. Rick.
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Fobes, J.F. Trisomic analysis of isozymic loci in tomato species: Segregation and dosage effects. Biochem Genet 18, 401–421 (1980). https://doi.org/10.1007/BF00484252
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DOI: https://doi.org/10.1007/BF00484252