Abstract
Adhnll is an ethyl methanesulfonate induced mutant that lacks detectable alcohol dehydrogenase activity. A number of indirect lines of evidence have indicated that the mutation responsible for this loss in enzyme activity is locoalized in the Adh structural gene. We present more direct evidence for this hypothesis here. Utilizing a newly developed procedure for comparing tryptic peptides of Drosophila alcohol dehydrogenase obtained from different strains, we show that the alcohol dehydrogenase-like protein isolated from Adh nll exhibits an altered peptide profile when compared to that of wild type. The implications of this finding as well as the utility of the method for attacking other genetic and developmental problems are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Benson, J. R. (1977). Improved ion-exchange resins. In Hirs, C. H. W., and Timasheff, S. N. (eds.), Methods in Enzmology, Vol. XLVII, Academic Press, New York.
Chovnick, A., Gelbart, W., and McCarron, M. (1977). Organization of the rosy locus in Drosophila melanogaster. Cell 111.
Dickinson, W. J., and Sullivan, D. T. (1975). Gene-enzyme systems in Drosophila. In Results and Problems in Cell Differentiation, Springer-Verlag, New York.
Grell, E. H., Jacobson, K. B., and Murphy, J. B. (1969). Alterations of genetic material for analysis of alcohol dehydrogenase isozymes of Drosophila melanogaster. Ann. N.Y. Acad. Sci. 151441.
Millman, G., Krauss, S.W., and Olsen, A.W. (1977). Tryptic peptide analysis of normal and mutant forms of hypoxanthine phosphoribosyltransferase from HeLa cells. Proc. Nat. Acad. Sci. USA 74926.
O'Donnell, J., Gerace, L., Leister, F., and Sofer, W. (1975). Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-penntyne-3-ol. Genetics 7973.
Paton, D.R., and Sullivan, D.T. (1978). Mutagenesis at the cinnibar locus in Drosophila melanogaster. Biochem. Genet. 16855.
Pelliccia, J.G., and Sofer, W. (1980) Manuscript in preparation.
Schwartz, M., and Jörnvall, H. (1976). Structural analysis of mutant and wild-type alcohol dehydrogenases from Drosophila melanogaster. Eur. J. Biochem. 68159.
Schwartz, M., and Sofer, W. (1976). Alcohol dehydrogenase-negative mutants in Drosophila:Defects at the structural locus? Genetics 83125.
Schwartz, M., Gerace, L., O'Donnell, J., and Sofer, W. (1975). Drosophila. In Third International Conference on Isozymes, Yale University, Vol. 1: Molecular Structure, Academic Press, New York.
Sofer, W., and Hatkoff, M.A. (1972). Chemical selection of alcohol dehydrogenase negative mutants in Drosophila. Genetics 72545.
Sofer, W., and Hatkoff, M.A. (1972). Chemical selection of alcohol dehydrogenase negative mutants in Drosophila. Genetics 72545.
Thatcher, D. R. (1977). Enzyme instability and proteolysis during the purification of an alcohol dehydrogenase from Drosophila melanogaster. Biochem. J. 163317.
Author information
Authors and Affiliations
Additional information
This work was supported by grants from the NIH (GM-18254 and ES-01527) and by a contract from the Department of Energy (EY-76-S-02-2965).
Contribution No. 1050 from the Department of Biology, John Hopkins University, Baltimore, Maryland 21218.
Rights and permissions
About this article
Cite this article
Reddy, A.R., Pelliccia, J.G. & Sofer, W. Adh-negative mutants: Detection of an altered tryptic peptide in a mutant enzyme of Drosophila . Biochem Genet 18, 339–351 (1980). https://doi.org/10.1007/BF00484247
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00484247