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Trace metal and vitamin requirements of Methanococcoides methylutens grown with trimethylamine

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Abstract

Trace organic nutrient and metal requirements for the growth of Methanococcoides methylutens strain TMA-10 were determined in defined medium that contained trimethylamine as the substrate. Biotin was the only organic supplement required in place of yeast extract, Trypticase or a mixture of 8 B-vitamins. Fe an Ni were required for growth and low concentrations of Fe2+ (<5 μM) and Ni2+ (<0.25 μM) provided limited growth. In the absence of added Co the growth rate was reduced by 94% and growth was limited at concentrations below 0.1 μM. Stimulation of growth by Se, Mo, B, Al, Zn, Mn or Cu could not be demonstrated.

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  1. Kevin R. Sowers

    Present address: Department of Microbiology, University of California, 5304 Life Sciences, 405 Hilgard Avenue, 90024, Los Angeles, CA, USA

Authors and Affiliations

  1. Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, 24061, Blacksburg, VA, USA

    Kevin R. Sowers & James G. Ferry

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  1. Kevin R. Sowers
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  2. James G. Ferry
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Sowers, K.R., Ferry, J.G. Trace metal and vitamin requirements of Methanococcoides methylutens grown with trimethylamine. Arch. Microbiol. 142, 148–151 (1985). https://doi.org/10.1007/BF00447058

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  • DOI: https://doi.org/10.1007/BF00447058

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