Abstract
Two plasmids, pAgK84::Tn5-Mob from Agrobacterium radiobacter carrying genes for the production of agrocin 84, and RP4-4 from E. coli were inserted either separately or together into a strain of Rhizobium meliloti. Each of these plasmid-containing R. meliloti transconjugants was less effective than the wild type strain in their ability to fix nitrogen in Medicago tornata. The pAgK84::Tn5-Mob-containing transconjugant was significantly less effective than that containing RP4-4. The transconjugant strains were inferior to the wild type strain in their ability to nodulate seedlings and to compete for nodulation.
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References
Beringer JE (1974) R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 84:188–198
Bhuvaneswari TV, Turgeon GB, Bauer WD (1980) Early events in the infection of soybean (Glycine max L. Merr) by Rhizobium japonicum. 1. Localization of infectible root cells. Plant Physiol 66:1027–1031
Hedges RW, Jacob AC (1974) Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet 132:31–40
Hendson M, Thompson JA (1986) Expression of an agrocinencoding plasmid of Agrobacterium tumefaciens in Rhizobium meliloti. J Appl Bacteriol 60:147–154
Hewitt EJ (1966) Sand and water culture methods used in the study of plant nutrition, 2nd edn. Commonwealth Agricultural Bureaux, Farnham Royal, England
Hynes MF, Bruksch K, Priefer U (1988) Melanin production encoded by a cryptic plasmid in a Rhizobium leguminosarum strain. Arch Microbiol 150:326–332
Kado CI, Liu S-T (1981) Rapid procedure for the detection of large and small plasmids. J Bacteriol 145:1365–1373
Kennedy C, Dreyfus B, Brockwell J (1981) Transfer, maintenance and expression of P plasmids in strains of cowpea rhizobia. J Gen Microbiol 125:233–240
Kerr A (1980) Biological control of crown gall through production of Agrocin 84. Plant Dis 64:25–30
Kerr A Htay K (1974) Biological control of crown gall through bacteriocin production. Physiol Plant Pathol 4:37–44
Miller J (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
Moore LW, Warren G (1979) Agrobacterium radiobacter strain 84 and biological control of crown gall. Ann Rev Phytopathol 7:163–179
Simon R (1984) High frequency mobilization of gram-negative bacterial replicons by the in vitro constructed Tn5-Mob transposon. Mol Gen Genet 196:413–420
Skot L, Hirsch PR, Witty JF (1986) Genetic factors in Rhizobium affecting the carbon costs of N2 fixation and host plant biomass production. J Appl Bacteriol 61:239–246
Spitzbarth M, Puhler A, Heumann W (1979) Characterization of plasmids isolated from Rhizobium meliloti. Arch Microbiol 121:1–7
Staphorst JL, van Zyl FGH, Strijdom BW, Grownewald ZE (1985) Agrocin-producing pathogenic and non-pathogenic biotype-3 strains of Agrobacterium tumefaciens active against biotype-3 pathogens. Curr Microbiol 13:45–42
Strobel GA, Lam B, Harrison L, Hess BM, Lam S (1985) Introduction of the hairy root plasmid into Rhizobium meliloti results in increased nodulation on its host. J Gen Microbiol 131:355–361
Strobel GA, Heide M, Hess BM (1986) Biology of Rhizobium trifolii bearing the hairy root plasmid. J Gen Microbiol 132:653–659
Vincent JM (1970) A manual for the practical study of root-nodule bacteria. Blackwell Scientific Publications, Oxford
Watson B, Currier TC, Gordon MP, Chilton M-D, Nester EW (1975) Plasmid required for virulence in Agrobacterium tumefaciens. J Bacteriol 123:255–264
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Law, I.J., Strijdom, B.W. Negative effects of agrocin 84-encoding Agrobacterium plasmids on symbiotic properties of Rhizobium meliloti . Arch. Microbiol. 152, 463–467 (1989). https://doi.org/10.1007/BF00446930
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DOI: https://doi.org/10.1007/BF00446930