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Isolation of auxotrophs and analysis of regulation of tryptophan biosynthesis in Zymomonas mobilis

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Abstract

Tryptophan auxotrophs were isolated and used to analyze the regulation of tryptophan biosynthesis in Zymomonas mobilis. Twelve tryptophan auxotrophs were cassified as trp E, B or A based on accumulation of, or growth on, indole and anthranilic acid. Trp B mutants were found to accumulate indole when grown on limiting, but not on excess tryptophan, suggesting that tryptophan plays a role in regulating its biosynthesis. Tryptophan synthase and indoleglycerol phosphate synthase specific activities were measured in the wild-type strain and two trp mutants grown in limiting or excess tryptophan. Neither activity was repressed by exogenous tryptophan.

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Abbreviations

CDRP:

O-(carboxyphenol amino)-1 deoxyribulose 5-phosphate

IGPS:

indoleglycerol phosphate synthase

TS:

tryptophan synthase

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Authors and Affiliations

  1. Department of Biology, Marquette University, 53233, Milwaukee, WI, USA

    Christina K. Eddy, K. Dale Noel & Oliver H. Smith

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  1. Christina K. Eddy
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  2. K. Dale Noel
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  3. Oliver H. Smith
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Dedicated in memory of Dr. O. H. Smith

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Eddy, C.K., Noel, K.D. & Smith, O.H. Isolation of auxotrophs and analysis of regulation of tryptophan biosynthesis in Zymomonas mobilis . Arch. Microbiol. 149, 561–564 (1988). https://doi.org/10.1007/BF00446761

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  • DOI: https://doi.org/10.1007/BF00446761

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